Cloning and production of a novel bacteriocin, lactococcin K, from Lactococcus lactis subsp lactis MY23

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A gene encoding the antimicrobial peptide, lactococcin K, was isolated from Lactococcus lactis subsp. lactis MY23 then cloned and expressed in Escherichia coli. Because the expressed lactococcin K was formed as an inclusion body in recombinant E. coli, a fusion protein containing lactococcin K and maltose-binding protein (MBP) was produced in a soluble form. For high-level production of lactococcin K, we performed a pH-stat fed-batch culture to produce 43,000 AU lactococcin K ml(-1) in 12 h.
Publisher
SPRINGER
Issue Date
2006-03
Language
English
Article Type
Article
Keywords

PEDIOCOCCUS-ACIDILACTICI; GENETIC-CHARACTERIZATION; ENTEROCOCCUS-FAECIUM; GEL-ELECTROPHORESIS; FOOD; PRESERVATION

Citation

BIOTECHNOLOGY LETTERS, v.28, pp.357 - 362

ISSN
0141-5492
DOI
10.1007/s10529-005-5935-z
URI
http://hdl.handle.net/10203/12478
Appears in Collection
BS-Journal Papers(저널논문)
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