Folate receptor-mediated intracellular delivery of recombinant caspase-3 for inducing apoptosis

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dc.contributor.authorCho, KCko
dc.contributor.authorJeong, JHko
dc.contributor.authorChung, Hyun Jungko
dc.contributor.authorJoe, Cheol Oko
dc.contributor.authorKim, SWko
dc.contributor.authorPark, Tae Gwanko
dc.date.accessioned2009-11-11T05:26:04Z-
dc.date.available2009-11-11T05:26:04Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2005-11-
dc.identifier.citationJOURNAL OF CONTROLLED RELEASE, v.108, no.1, pp.121 - 131-
dc.identifier.issn0168-3659-
dc.identifier.urihttp://hdl.handle.net/10203/12414-
dc.description.abstractR ecombinant reversed caspase-3 (rev-caspase-3) is a pro-apoptotic gene capable of intracellular autocatalytic processing, which leads to programmed cell death. Folate receptor-specific intracellular delivery of the rev-caspase-3 gene into KB cells overexpressing folate receptors was explored by employing the folate-poly(ethylene glycol)-polyethylenimine (FOL-PEG-PEI) conjugate as a nonviral polymeric carrier. Using luciferase as a reporter gene, the conditions for formulation of DNA/polymer polyplexes were pre-optimized to attain the highest folate receptor-mediated gene transfection efficiency. FOL-PEG-PEI conjugate complexed with rev-caspase-3 plasmid in an optimized condition gave rise to a great increase in expression and activation of exogenous rev-caspase-3 in KB cells when pretreated with doxorubicin. The synthesized conjugate exhibited higher transfection efficiency than other commercially available transfection agents due to a unique mechanism of folate-receptor mediated endocytic gene transfer. The transfected cells showed a significant extent of apoptosis by rev-caspase-3. This study suggests the potential of using folate-receptor-mediated delivery of rev-caspase-3 gene for inducing tumor cell death in a target-specific manner. (c) 2005 Elsevier B.V All rights reserved.-
dc.description.sponsorshipNational Cancer Institute (02-3-150) and from the Ministry of Science and Technology (M102140000117- 02b1500-02110), Republic of Korea.en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherELSEVIER SCIENCE BV-
dc.subjectGENE DELIVERY-
dc.subjectCATIONIC POLYMERS-
dc.subjectPLASMID DNA-
dc.subjectCELLS-
dc.subjectCANCER-
dc.subjectPOLYETHYLENIMINE-
dc.subjectBINDING-
dc.subjectGA-67-DEFEROXAMINE-FOLATE-
dc.subjectEXPRESSION-
dc.subjectCOMPLEXES-
dc.titleFolate receptor-mediated intracellular delivery of recombinant caspase-3 for inducing apoptosis-
dc.typeArticle-
dc.identifier.wosid000233321200012-
dc.identifier.scopusid2-s2.0-26944466093-
dc.type.rimsART-
dc.citation.volume108-
dc.citation.issue1-
dc.citation.beginningpage121-
dc.citation.endingpage131-
dc.citation.publicationnameJOURNAL OF CONTROLLED RELEASE-
dc.identifier.doi10.1016/j.jconrel.2005.07.015-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorChung, Hyun Jung-
dc.contributor.localauthorJoe, Cheol O-
dc.contributor.localauthorPark, Tae Gwan-
dc.contributor.nonIdAuthorCho, KC-
dc.contributor.nonIdAuthorJeong, JH-
dc.contributor.nonIdAuthorKim, SW-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorPEI-
dc.subject.keywordAuthorfolate-
dc.subject.keywordAuthorreceptor-mediated endocytosis-
dc.subject.keywordAuthorrev-caspase-3-
dc.subject.keywordAuthorapoptosis-
dc.subject.keywordPlusGENE DELIVERY-
dc.subject.keywordPlusCATIONIC POLYMERS-
dc.subject.keywordPlusPLASMID DNA-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusCANCER-
dc.subject.keywordPlusPOLYETHYLENIMINE-
dc.subject.keywordPlusBINDING-
dc.subject.keywordPlusGA-67-DEFEROXAMINE-FOLATE-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusCOMPLEXES-
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