Polymeric Rulers: Distance-Dependent Emission Behaviors of Fluorophores on Flat Gold Surfaces and Bioassay Platforms Using Plasmonic Fluorescence Enhancement

Cited 34 time in webofscience Cited 35 time in scopus
  • Hit : 296
  • Download : 29
In this paper, we introduce the concept of a "polymeric ruler" for investigating distance-dependent emission behaviors of fluorophores, namely the quenching or enhancement of fluorescence, on flat Au surfaces in the range of 5 to similar to 100 nm, which has not previously been easily accessible. The polymeric ruler is constructed by a highly controllable surface-initiated atom transfer radical polymerization of oligo(ethylene glycol)methacrylate (OEGMA), and the obtained thicknesses of the poly(OEGMA) (pOEGMA) layers range from similar to 5 to similar to 80 nm. The quenching or enhancement of fluorescence is found to be dependent upon the distance between fluorophores and the Au surface. In brief, fluorescence quenching occurs at distances within about 15 nm from the Au surface, and surface-enhanced fluorescence is observed at tens of nanometers beyond the range of quenching with the maximum enhancement at about 40-50 nm. The obtained information on the distance-dependent surface-enhanced fluorescence is applied to the construction of highly sensitive bioassay platforms: the use of the 50 nm thick pOEGMA layer lowers the detection limit up to 1 pM.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2008-11
Language
English
Article Type
Article
Keywords

TRANSFER RADICAL POLYMERIZATION; DYE-LABELED OLIGONUCLEOTIDES; SILVER NANOPARTICLES; MOLECULAR FLUORESCENCE; BRUSHES; PROTEIN; MONOLAYERS; FILMS; LUMINESCENCE; SPECTROSCOPY

Citation

ADVANCED FUNCTIONAL MATERIALS, v.18, no.21, pp.3395 - 3402

ISSN
1616-301X
DOI
10.1002/adfm.200800471
URI
http://hdl.handle.net/10203/11993
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 34 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0