DC Field | Value | Language |
---|---|---|
dc.contributor.author | TAE GWAN PARK | ko |
dc.contributor.author | Lee, H | ko |
dc.contributor.author | Kim, TH | ko |
dc.date.accessioned | 2009-09-07T05:57:06Z | - |
dc.date.available | 2009-09-07T05:57:06Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2002-09 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v.83, no.1, pp.109 - 119 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/10203/11045 | - |
dc.description.abstract | An efficient receptor-mediated non-viral gene delivery formulation based on mono-pegylated recombinant human epidermal growth factor (EGF) was developed using a streptavidin-biotin system. Biotin-derivatized and mono-pegylated EGF was prepared by conjugating a biotin-PEG-NHS derivative to EGF and purified through a chromatographic method. Luciferase plasmid DNA and polyethylenimine (PEI) were complexed to form positively charged nanoparticles on which negatively charged streptavidin was first coated and then biotin-PEG-EGF conjugate was immobilized via streptavidin-biotin interaction. The EGF-PEG-biotin-streptavidin-PEI-DNA complexes were characterized in terms of their effective diameter and surface zeta (zeta)-potential value under various formulation conditions. The formulated complexes exhibited high transfection efficiency (similar to10(8) in luciferase activity) with no inter-particle aggregation. This was attributed to enhanced cellular uptake of the resultant complexes via receptor-mediated endocytosis. Furthermore, in the presence of serum proteins, a slight decrease in transfection efficiency was observed due to the presence of PEG chains on the surface. (C) 2002 Elsevier Science B.V. All rights reserved. | - |
dc.description.sponsorship | the Ministry of Health and Welfare, South Korea | en |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | Elsevier Science Bv | - |
dc.subject | BLOCK-COPOLYMER | - |
dc.subject | T-LYMPHOCYTES | - |
dc.subject | CELLS | - |
dc.subject | DNA | - |
dc.subject | TRANSFECTION | - |
dc.subject | EXPRESSION | - |
dc.subject | BINDING | - |
dc.subject | PEPTIDE | - |
dc.subject | RESISTANCE | - |
dc.subject | INHIBITION | - |
dc.title | A Receptor-mediated Gene Delivery System Using Streptavidin and Biotin-derivatized, Pegylated Epidermal Growth Factor | - |
dc.type | Article | - |
dc.identifier.wosid | 000178566300011 | - |
dc.identifier.scopusid | 2-s2.0-0037130561 | - |
dc.type.rims | ART | - |
dc.citation.volume | 83 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 109 | - |
dc.citation.endingpage | 119 | - |
dc.citation.publicationname | JOURNAL OF CONTROLLED RELEASE | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | TAE GWAN PARK | - |
dc.contributor.nonIdAuthor | Lee, H | - |
dc.contributor.nonIdAuthor | Kim, TH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | receptor-mediated gene delivery | - |
dc.subject.keywordAuthor | EGF | - |
dc.subject.keywordAuthor | polyethylene glycol (PEG) | - |
dc.subject.keywordAuthor | streptavidin-biotin | - |
dc.subject.keywordPlus | BLOCK-COPOLYMER | - |
dc.subject.keywordPlus | T-LYMPHOCYTES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | TRANSFECTION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | RESISTANCE | - |
dc.subject.keywordPlus | INHIBITION | - |
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