DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SB | - |
dc.contributor.author | Chae, GW | - |
dc.contributor.author | Lee, J | - |
dc.contributor.author | Park, J | - |
dc.contributor.author | Tak, H | - |
dc.contributor.author | Chung, JH | - |
dc.contributor.author | Park, TG | - |
dc.contributor.author | Ahn, JK | - |
dc.contributor.author | Joe, CO | - |
dc.date.accessioned | 2009-08-24T06:02:16Z | - |
dc.date.available | 2009-08-24T06:02:16Z | - |
dc.date.issued | 2007-05-14 | - |
dc.identifier.citation | Cell Death Differentiation, Vol.14, pp.982-991 | en |
dc.identifier.uri | http://hdl.handle.net/10203/10709 | - |
dc.description.abstract | We propose a biochemical mechanism for the negative role of Notch signaling on p53 transactivation function. Expression of the intracellular domain of human Notch1 (Notch1-IC) inhibits the expression of p53-responsive genes p21, mdm2, and bax in HCT116 p53-/- cells. Furthermore, Notch1-IC expression inhibits the phosphorylation of ectopically expressed p53 in HCT116 p53-/- cells as well as the phosphorylation of endogenous p53 in UV-treated HCT116 p53+/+ cells. Transcriptional downregulation of p53-responsive genes by Notch1-IC was confirmed both by chromatin immunoprecipitation assay and Northern blot analysis. We found the intracellular interaction between Notch1-IC and p53 in HCT116 p53+/+ cells and suggest that activated Notch1 interaction with p53 is an important cellular event for the inhibition of p53-dependent transactivation. The N-terminal fragment of Notch1-IC, which can interacts with p53, inhibits p53 phosphorylation and represses p53 transactivation. In addition, Notch signaling downregulated p53-dependent apoptosis induced by UV irradiation. | en |
dc.language.iso | en_US | en |
dc.publisher | Nature Publishing Group | en |
dc.subject | p53 | en |
dc.subject | Notch | en |
dc.subject | apoptosis | en |
dc.subject | phosphorylation | en |
dc.title | Activated Notch1 interacts with p53 to inhibit its phosphorylation and transactivation | en |
dc.type | Article | en |
dc.identifier.doi | 10.1038/sj.cdd.4402083 | - |
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