DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Tae-Gwan | ko |
dc.date.accessioned | 2009-08-12T05:23:49Z | - |
dc.date.available | 2009-08-12T05:23:49Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 1993 | - |
dc.identifier.citation | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v.4, no.5, pp.493 - 504 | - |
dc.identifier.issn | 0920-5063 | - |
dc.identifier.uri | http://hdl.handle.net/10203/10513 | - |
dc.description.abstract | The enzyme, alkaline phosphatase, has been conjugated to a temperature-sensitive polymer which exhibits a lower critical solution temperature (LCST). A series of copolymers containing different molar ratios of N-isopropylacrylamide(NIPAAm) and N-acryloxysuccinimide(NAS) were synthesized and then conjugated to the enzyme. These polymer-enzyme conjugates precipitate and flocculate in aqueous solution above the LCST, and redissolve when cooled below that temperature. The kinetics of the conjugated enzymes have been characterized as a function of temperature and compared to free enzyme. The effect of the conjugation degree between polymer and enzyme on the activity of the conjugated enzymes was also investigated. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | VSP BV | - |
dc.subject | POLYETHYLENE-GLYCOL | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | STABILIZATION | - |
dc.subject | PROTEINS | - |
dc.title | SYNTHESIS AND CHARACTERIZATION OF A SOLUBLE, TEMPERATURE-SENSITIVE POLYMER-CONJUGATED ENZYME | - |
dc.type | Article | - |
dc.identifier.wosid | A1993LU04000008 | - |
dc.identifier.scopusid | 2-s2.0-0027357207 | - |
dc.type.rims | ART | - |
dc.citation.volume | 4 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 493 | - |
dc.citation.endingpage | 504 | - |
dc.citation.publicationname | JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Park, Tae-Gwan | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CONJUGATED | - |
dc.subject.keywordAuthor | ENZYME | - |
dc.subject.keywordAuthor | ALKALINE PHOSPHATASE | - |
dc.subject.keywordPlus | POLYETHYLENE-GLYCOL | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | PROTEINS | - |
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