Really Smart Bioconjugates of Smart Polymers and Receptor Proteins

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Over the past 18 years we have been deeply involved with the synthesis and applications of stimuli-responsive polymer systems, especially polymer-biomolecule conjugates. This article summarizes our work with one of these conjugate systems, specifically polymer-protein conjugates. We include conjugates prepared by random polymer conjugation to lysine amino groups, and also those prepared by site-specific conjugation of the polymer to specific amino acid sites that are genetically engineered into the known amino acid sequence of the protein. We describe the preparation and properties of thermally sensitive random conjugates to enzymes and several affinity recognition proteins. We have also prepared site-specific conjugates to streptavidin with temperature-sensitive polymers, pH-sensitive polymers, and light-sensitive polymers. The preparation of these conjugates and their many fascinating applications are reviewed in this article. (C) 2000 John Wiley & Sons, Inc.
Publisher
Wiley-Blackwell
Issue Date
2000-12
Language
English
Article Type
Article
Keywords

AFFINITY PRECIPITATION; N-ISOPROPYLACRYLAMIDE; RESPONSIVE POLYMER; THERMOPRECIPITATION; STREPTAVIDIN; PURIFICATION; CONJUGATION; ADVANTAGES; CELLULASE; ENZYMES

Citation

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, v.52, no.4, pp.577 - 586

ISSN
1549-3296
DOI
10.1002/1097-4636(20001215)52:4<577::AID-JBM1>3.0.CO;2-5
URI
http://hdl.handle.net/10203/10508
Appears in Collection
BS-Journal Papers(저널논문)
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