An application of beta-glycosidase to transformation of ginsenosides for the effective production of specific ginsenosides with biological efficacy

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Over the past several decades, the pharmacological effects of ginsenosides in Panax ginseng roots have been extensively investigated. Here, we developed a method for producing specific ginsenosides (F1 and F2) with good yields (F1:162 mg/g, F2:305 mg/g) using beta-glycosidase purified from Aspergillus niger. In addition, each ginsenoside (at least 25 species) was separated and purified by high performance liquid chromatography (HPLC) using five different types of solvents and different purification steps. In addition, the Rg3:Rh2 mixture (1:1, w/w) was shown to inhibit a specific lung cancer cell line (NCI-H232) in vivo, displaying an anticancer effect at a dose lower than achieved using treatments with single Rg3 or Rh2. This finding suggests that the combination of ginsenosides for targeting anticancer is more effective than the use of a single ginsenoside from ginseng or red ginseng.
Publisher
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
Issue Date
2012-06
Language
Korean
Article Type
Article
Keywords

COMPOUND-K; ENZYMATIC PREPARATION; ANTIALLERGIC ACTIVITY; GINSENG SAPONIN; PROSTATE-CANCER; PANAX-GINSENG; CELLS; CONSTITUENTS; METABOLITE; INDUCTION

Citation

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.17, no.3, pp.538 - 546

ISSN
1226-8372
DOI
10.1007/s12257-011-0678-2
URI
http://hdl.handle.net/10203/104235
Appears in Collection
CBE-Journal Papers(저널논문)
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