DC Field | Value | Language |
---|---|---|
dc.contributor.author | Nam, Eun-Hee | ko |
dc.contributor.author | Lee, Yunhee | ko |
dc.contributor.author | Park, Young-Kyu | ko |
dc.contributor.author | Lee, Jung Weon | ko |
dc.contributor.author | Kim, Semi | ko |
dc.date.accessioned | 2013-03-12T22:58:33Z | - |
dc.date.available | 2013-03-12T22:58:33Z | - |
dc.date.created | 2013-01-09 | - |
dc.date.created | 2013-01-09 | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | CARCINOGENESIS, v.33, no.3, pp.563 - 571 | - |
dc.identifier.issn | 0143-3334 | - |
dc.identifier.uri | http://hdl.handle.net/10203/103791 | - |
dc.description.abstract | Epithelial-mesenchymal transition (EMT) is a process implicated in tumor invasion, metastasis, embryonic development and wound healing. ZEB2 is a transcription factor involved in EMT that represses E-cadherin transcription. Although E-cadherin downregulation is a major event during EMT and tumor progression, E-cadherin reduction is probably not sufficient for full invasiveness. The mechanisms by which E-cadherin transcriptional repressors induce mesenchymal genes during EMT remain largely unknown. Here, we investigated the role of ZEB2 in the induction of integrin alpha 5 during cancer EMT and its underlying mechanism. In human cancer cells, ZEB2 was found to directly upregulate integrin alpha 5 transcription in a manner that is independent of the regulation of E-cadherin expression. Conversely, depletion of ZEB2 by small interfering RNA suppressed integrin alpha 5 expression, leading to reduced invasion. Suppression of integrin alpha 5 inhibited cancer cell invasion, suggesting an important role for integrin alpha 5 in cancer progression. Furthermore, ZEB2 was found to activate the integrin alpha 5 and vimentin promoters by interacting with and activating the transcription factor Sp1, suggesting that cooperation between ZEB2 and Sp1 represents a novel mechanism of mesenchymal gene activation during EMT. These findings increase our understanding of the pathways beyond E-cadherin reduction that regulate mesenchymal gene expression during EMT and cancer progression. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | TRANSCRIPTION FACTORS | - |
dc.subject | TUMOR-CELLS | - |
dc.subject | CARCINOMA-CELLS | - |
dc.subject | GENE PROMOTER | - |
dc.subject | E-CADHERIN | - |
dc.subject | TGF-BETA | - |
dc.subject | METASTASIS | - |
dc.subject | FIBRONECTIN | - |
dc.subject | BINDING | - |
dc.subject | SNAIL | - |
dc.title | ZEB2 upregulates integrin alpha 5 expression through cooperation with Sp1 to induce invasion during epithelial-mesenchymal transition of human cancer cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000300988100011 | - |
dc.identifier.scopusid | 2-s2.0-84863236117 | - |
dc.type.rims | ART | - |
dc.citation.volume | 33 | - |
dc.citation.issue | 3 | - |
dc.citation.beginningpage | 563 | - |
dc.citation.endingpage | 571 | - |
dc.citation.publicationname | CARCINOGENESIS | - |
dc.identifier.doi | 10.1093/carcin/bgs005 | - |
dc.contributor.nonIdAuthor | Nam, Eun-Hee | - |
dc.contributor.nonIdAuthor | Lee, Yunhee | - |
dc.contributor.nonIdAuthor | Park, Young-Kyu | - |
dc.contributor.nonIdAuthor | Lee, Jung Weon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | TRANSCRIPTION FACTORS | - |
dc.subject.keywordPlus | TUMOR-CELLS | - |
dc.subject.keywordPlus | CARCINOMA-CELLS | - |
dc.subject.keywordPlus | GENE PROMOTER | - |
dc.subject.keywordPlus | E-CADHERIN | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | METASTASIS | - |
dc.subject.keywordPlus | FIBRONECTIN | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | SNAIL | - |
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