DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jung, Dong-Jun | ko |
dc.contributor.author | An, Jang-Hyun | ko |
dc.contributor.author | Kurokawa, Kenji | ko |
dc.contributor.author | Jung, Yoon-Chuel | ko |
dc.contributor.author | Kim, Min-Jung | ko |
dc.contributor.author | Aoyagi, Youko | ko |
dc.contributor.author | Matsushita, Misao | ko |
dc.contributor.author | Takahashi, Shinji | ko |
dc.contributor.author | Lee, Hee-Seung | ko |
dc.contributor.author | Takahashi, Kazue | ko |
dc.date.accessioned | 2013-03-12T19:57:51Z | - |
dc.date.available | 2013-03-12T19:57:51Z | - |
dc.date.created | 2012-12-10 | - |
dc.date.created | 2012-12-10 | - |
dc.date.issued | 2012-11 | - |
dc.identifier.citation | JOURNAL OF IMMUNOLOGY, v.189, no.10, pp.4951 - 4959 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | http://hdl.handle.net/10203/103356 | - |
dc.description.abstract | Wall teichoic acid (WTA) of Staphylococcus aureus is a major cell envelope-associated glycopolymer that is a key molecule in promoting colonization during S. aureus infection. The complement system plays a key role in the opsonization and clearance of pathogens. We recently reported that S. aureus WTA functions as a ligand of human serum mannose-binding lectin (MBL), a recognition molecule of the lectin complement pathway. Intriguingly, serum MBL in adults does not bind to WTA because of an inhibitory effect of serum anti-WTA-IgG. In this study, serum anti-WTA-IgG was purified to homogeneity using a purified S. aureus WTA-coupled affinity column to examine the biological function of human anti-WTA-IgG. The purified anti-WTA-IgG contained the IgG2 subclass as a major component and specifically induced C4 and C3 deposition on the S. aureus surface in the anti-WTA-IgG-depleted serum, but not in C1q-deficient serum. Furthermore, the anti-WTA-IgG-dependent C3 deposition induced phagocytosis of S. aureus cells by human polymorphonuclear leukocytes. These results demonstrate that serum anti-WTA-IgG is a real trigger for the induction of classical complement-dependent opsonophagocytosis against S. aureus. Our results also support the fact that a lack of the lectin complement pathway in MBL-deficient adults is compensated by Ag-specific, Ab-mediated adaptive immunity. The Journal of Immunology, 2012, 189: 4951-4959. | - |
dc.language | English | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.subject | MANNOSE-BINDING LECTIN | - |
dc.subject | GRAM-POSITIVE BACTERIA | - |
dc.subject | LIPOTEICHOIC ACID | - |
dc.subject | HUMAN POLYMORPHONUCLEAR | - |
dc.subject | SERINE-PROTEASE | - |
dc.subject | 2 PARTS | - |
dc.subject | ACTIVATION | - |
dc.subject | OPSONIZATION | - |
dc.subject | INFECTIONS | - |
dc.subject | ANTIBODIES | - |
dc.title | Specific Serum Ig Recognizing Staphylococcal Wall Teichoic Acid Induces Complement-Mediated Opsonophagocytosis against Staphylococcus aureus | - |
dc.type | Article | - |
dc.identifier.wosid | 000310710600029 | - |
dc.identifier.scopusid | 2-s2.0-84868524290 | - |
dc.type.rims | ART | - |
dc.citation.volume | 189 | - |
dc.citation.issue | 10 | - |
dc.citation.beginningpage | 4951 | - |
dc.citation.endingpage | 4959 | - |
dc.citation.publicationname | JOURNAL OF IMMUNOLOGY | - |
dc.identifier.doi | 10.4049/jimmunol.1201294 | - |
dc.contributor.localauthor | Lee, Hee-Seung | - |
dc.contributor.nonIdAuthor | Jung, Dong-Jun | - |
dc.contributor.nonIdAuthor | An, Jang-Hyun | - |
dc.contributor.nonIdAuthor | Kurokawa, Kenji | - |
dc.contributor.nonIdAuthor | Kim, Min-Jung | - |
dc.contributor.nonIdAuthor | Aoyagi, Youko | - |
dc.contributor.nonIdAuthor | Matsushita, Misao | - |
dc.contributor.nonIdAuthor | Takahashi, Shinji | - |
dc.contributor.nonIdAuthor | Takahashi, Kazue | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | MANNOSE-BINDING LECTIN | - |
dc.subject.keywordPlus | GRAM-POSITIVE BACTERIA | - |
dc.subject.keywordPlus | LIPOTEICHOIC ACID | - |
dc.subject.keywordPlus | HUMAN POLYMORPHONUCLEAR | - |
dc.subject.keywordPlus | SERINE-PROTEASE | - |
dc.subject.keywordPlus | 2 PARTS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | OPSONIZATION | - |
dc.subject.keywordPlus | INFECTIONS | - |
dc.subject.keywordPlus | ANTIBODIES | - |
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