Specific Serum Ig Recognizing Staphylococcal Wall Teichoic Acid Induces Complement-Mediated Opsonophagocytosis against Staphylococcus aureus

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Wall teichoic acid (WTA) of Staphylococcus aureus is a major cell envelope-associated glycopolymer that is a key molecule in promoting colonization during S. aureus infection. The complement system plays a key role in the opsonization and clearance of pathogens. We recently reported that S. aureus WTA functions as a ligand of human serum mannose-binding lectin (MBL), a recognition molecule of the lectin complement pathway. Intriguingly, serum MBL in adults does not bind to WTA because of an inhibitory effect of serum anti-WTA-IgG. In this study, serum anti-WTA-IgG was purified to homogeneity using a purified S. aureus WTA-coupled affinity column to examine the biological function of human anti-WTA-IgG. The purified anti-WTA-IgG contained the IgG2 subclass as a major component and specifically induced C4 and C3 deposition on the S. aureus surface in the anti-WTA-IgG-depleted serum, but not in C1q-deficient serum. Furthermore, the anti-WTA-IgG-dependent C3 deposition induced phagocytosis of S. aureus cells by human polymorphonuclear leukocytes. These results demonstrate that serum anti-WTA-IgG is a real trigger for the induction of classical complement-dependent opsonophagocytosis against S. aureus. Our results also support the fact that a lack of the lectin complement pathway in MBL-deficient adults is compensated by Ag-specific, Ab-mediated adaptive immunity. The Journal of Immunology, 2012, 189: 4951-4959.
Publisher
AMER ASSOC IMMUNOLOGISTS
Issue Date
2012-11
Language
English
Article Type
Article
Keywords

MANNOSE-BINDING LECTIN; GRAM-POSITIVE BACTERIA; LIPOTEICHOIC ACID; HUMAN POLYMORPHONUCLEAR; SERINE-PROTEASE; 2 PARTS; ACTIVATION; OPSONIZATION; INFECTIONS; ANTIBODIES

Citation

JOURNAL OF IMMUNOLOGY, v.189, no.10, pp.4951 - 4959

ISSN
0022-1767
DOI
10.4049/jimmunol.1201294
URI
http://hdl.handle.net/10203/103356
Appears in Collection
CH-Journal Papers(저널논문)
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