Polydopamine-mediated surface modification of scaffold materials for human neural stem cell engineering

Cited 300 time in webofscience Cited 0 time in scopus
  • Hit : 558
  • Download : 1469
Surface modification of tissue engineering scaffolds and substrates is required for improving the efficacy of stem cell therapy by generating physicochemical stimulation promoting proliferation and differentiation of stem cells. However, typical surface modification methods including chemical conjugation or physical absorption have several limitations such as multistep, complicated procedures, surface denaturation, batch-to-batch inconsistencies, and low surface conjugation efficiency. In this study, we report a mussel-inspired, biomimetic approach to surface modification for efficient and reliable manipulation of human neural stem cell (NSC) differentiation and proliferation. Our study demonstrates that polydopamine coating facilitates highly efficient, simple immobilization of neurotrophic growth factors and adhesion peptides onto polymer substrates. The growth factor or peptide-immobilized substrates greatly enhance differentiation and proliferation of human NSCs (human fetal brain-derived NSCs and human induced pluripotent stem cell-derived NSCs) at a level comparable or greater than currently available animal-derived coating materials (Matrigel) with safety issues. Therefore, polydopamine-mediated surface modification can provide a versatile platform technology for developing chemically defined, safe, functional substrates and scaffolds for therapeutic applications of human NSCs. (C) 2012 Elsevier Ltd. All rights reserved.
Publisher
ELSEVIER SCI LTD
Issue Date
2012-10
Language
English
Article Type
Article
Keywords

NEURONAL DIFFERENTIATION; IN-VITRO; ENDOTHELIAL-CELLS; PRECURSOR CELLS; GROWTH-FACTOR; TRANSPLANTATION; SURVIVAL; STROKE; PROLIFERATION; VIABILITY

Citation

BIOMATERIALS, v.33, no.29, pp.6952 - 6964

ISSN
0142-9612
DOI
10.1016/j.biomaterials.2012.06.067
URI
http://hdl.handle.net/10203/103297
Appears in Collection
CH-Journal Papers(저널논문)
Files in This Item
000308269600006.pdf(2.78 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 300 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0