DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, Seung_Wook | ko |
dc.contributor.author | Choi, Kyungsun | ko |
dc.contributor.author | Park, Jong-Hwan | ko |
dc.contributor.author | Park, Yeong-Min | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Choi, Chulhee | ko |
dc.date.accessioned | 2013-03-12T17:17:12Z | - |
dc.date.available | 2013-03-12T17:17:12Z | - |
dc.date.created | 2012-10-09 | - |
dc.date.created | 2012-10-09 | - |
dc.date.issued | 2012-10 | - |
dc.identifier.citation | CANCER LETTERS, v.323, no.1, pp.62 - 68 | - |
dc.identifier.issn | 0304-3835 | - |
dc.identifier.uri | http://hdl.handle.net/10203/102978 | - |
dc.description.abstract | Mitochondrial fusion and fission are dynamically regulated during apoptotic cell death, and mitofusin (Mfn) and related proteins have been shown to be involved in apoptosis-associated changes in mitochondrial morphology and function. Here, we investigated the involvement of Mfn proteins in the conformational activation and mitochondrial translocation of Bax, a key molecule responsible for apoptosis-associated mitochondrial changes. When ectopically expressed, Mfn1 inhibited the amino-terminal activation, but not the mitochondrial translocation, of Bax during staurosporine-induced apoptosis; overexpression of Mfn2 had no effect. Overexpression of Mfn1 mutants carrying point mutations in the GTPase domain (Mfn1-1038T and Mfn1-T109A) did not inhibit the amino-terminal activation of Bax. Furthermore, staurosporine-induced amino-terminal activation of Bax was significantly delayed in Mfn1-shRNA transfected (Mfn1-depleted) HeLa cells compared to cells transfected with control shRNA. These results collectively suggest a role for Mfn1 in regulating the activation of Bax on the outer mitochondrial membrane in a GTPase-dependent manner. (C) 2012 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.subject | CYTOCHROME-C RELEASE | - |
dc.subject | INDUCED CELL-DEATH | - |
dc.subject | ENDOPLASMIC-RETICULUM | - |
dc.subject | MAMMALIAN-CELLS | - |
dc.subject | FUSION | - |
dc.subject | FISSION | - |
dc.subject | PROTEIN | - |
dc.subject | DYNAMICS | - |
dc.subject | ACTIVATION | - |
dc.subject | MORPHOLOGY | - |
dc.title | Mitofusin 1 inhibits an apoptosis-associated amino-terminal conformational change in Bax, but not its mitochondrial translocation, in a GTPase-dependent manner | - |
dc.type | Article | - |
dc.identifier.wosid | 000305721000008 | - |
dc.identifier.scopusid | 2-s2.0-84861823403 | - |
dc.type.rims | ART | - |
dc.citation.volume | 323 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 62 | - |
dc.citation.endingpage | 68 | - |
dc.citation.publicationname | CANCER LETTERS | - |
dc.identifier.doi | 10.1016/j.canlet.2012.03.038 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.localauthor | Choi, Chulhee | - |
dc.contributor.nonIdAuthor | Choi, Kyungsun | - |
dc.contributor.nonIdAuthor | Park, Jong-Hwan | - |
dc.contributor.nonIdAuthor | Park, Yeong-Min | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | Bax | - |
dc.subject.keywordAuthor | Mitochondria | - |
dc.subject.keywordAuthor | Mitofusin | - |
dc.subject.keywordAuthor | Mfn1 | - |
dc.subject.keywordAuthor | Mfn2 | - |
dc.subject.keywordAuthor | Outer mitochondrial membrane | - |
dc.subject.keywordPlus | CYTOCHROME-C RELEASE | - |
dc.subject.keywordPlus | INDUCED CELL-DEATH | - |
dc.subject.keywordPlus | ENDOPLASMIC-RETICULUM | - |
dc.subject.keywordPlus | MAMMALIAN-CELLS | - |
dc.subject.keywordPlus | FUSION | - |
dc.subject.keywordPlus | FISSION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MORPHOLOGY | - |
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