Experimental phasing using zinc anomalous scattering

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Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.
Publisher
WILEY-BLACKWELL
Issue Date
2012-09
Language
English
Article Type
Article
Keywords

EXTENDED SUBSTRATE SPECTRUM; STRUCTURAL BASIS; CRYSTAL-STRUCTURE; BINDING; RECOGNITION; MODEL; SPECIFICITY; ACTIVATION; REFINEMENT; RECEPTORS

Citation

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v.68, pp.1253 - 1258

ISSN
0907-4449
DOI
10.1107/S0907444912024420
URI
http://hdl.handle.net/10203/102973
Appears in Collection
BS-Journal Papers(저널논문)
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