Increasing demands on fossil fuel have led to the unprecedented attraction to microalgal biofuel as an alternative
energy. In this study, we investigated growth and lipid productions of microalga Nannochloris oculata under various
carbon dioxide or nitrogen source concentrations and irradiance conditions. Biomass production of N. oculata was
highest under 2% CO2 with 0.3 flow rate (vvm). In addition, biomass productivities were proportional to the concentration
of nitrogen source, whereas lipid biosynthesis was suppressed under higher nitrogen concentration (up to 50 mg/L).
High irradiation (160~180 μmol/m2·s) enhanced growth rate and lipid production of N. oculata.