An electrically active microneedle array for electroporation

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We have designed and fabricated a microneedle array with electrical functionality with the final goal of electroporating skin's epidermal cells to increase their transfection by DNA vaccines. The microneedle array was made of polymethylmethacrylate (PMMA) by micromolding technology from a polydimethylsiloxane (PDMS) mold, followed by metal deposition, patterning using laser ablation, and electrodeposition. This microneedle array possessed sufficient mechanical strength to penetrate human skin in vivo and was also able to electroporate both red blood cells and human prostate cancer cells as an in vitro model to demonstrate cell membrane permeabilization. A computational model to predict the effective volume for electroporation with respect to applied voltages was constructed from finite element simulation. This study demonstrates the mechanical and electrical functionalities of the first MEMS-fabricated microneedle array for electroporation, designed for DNA vaccine delivery.
Publisher
SPRINGER
Issue Date
2010-04
Language
English
Article Type
Article
Keywords

TRANSDERMAL DRUG-DELIVERY; DNA VACCINES; GENE-TRANSFER; IN-VIVO; ENHANCED DELIVERY; SKIN; IMMUNIZATION; SYSTEM; ELECTROCHEMOTHERAPY; MICROFABRICATION

Citation

BIOMEDICAL MICRODEVICES, v.12, no.2, pp.263 - 273

ISSN
1387-2176
DOI
10.1007/s10544-009-9381-x
URI
http://hdl.handle.net/10203/102661
Appears in Collection
CBE-Journal Papers(저널논문)
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