DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Jinho | ko |
dc.contributor.author | Kim, Sunghyun | ko |
dc.contributor.author | Saw, Phei Er | ko |
dc.contributor.author | Lee, In-Hyun | ko |
dc.contributor.author | Yu, Mi Kyung | ko |
dc.contributor.author | Kim, Minsik | ko |
dc.contributor.author | Lee, Kwangyeol | ko |
dc.contributor.author | Kim, Yong-Chul | ko |
dc.contributor.author | Jeong, Yong Yeon | ko |
dc.contributor.author | Jon, Sangyong | ko |
dc.date.accessioned | 2013-03-12T14:44:22Z | - |
dc.date.available | 2013-03-12T14:44:22Z | - |
dc.date.created | 2012-10-30 | - |
dc.date.created | 2012-10-30 | - |
dc.date.issued | 2012-10 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, v.163, no.2, pp.111 - 118 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://hdl.handle.net/10203/102628 | - |
dc.description.abstract | Fibronectin extra domain B (EDB) is specifically expressed in cancer-associated blood vessels and extracellular matrix, and thus is a promising cancer biomarker. Very recently, we developed a novel class of high-affinity (<100 nM) peptides, termed 'aptides', that specifically bind a variety of protein targets. Here, we describe superparamagnetic iron oxide nanoparticles (SPIONs) conjugated with EDB-specific aptides for use in targeted magnetic resonance imaging (MRI) of cancer. An anti-EDB aptide (APT(EDB)) containing an additional cysteine residue reacted with maleimide-terminated, PEGylated phospholipid (Mal-PEG(2000)-DSPE) to give an aptide-conjugated PEGylated phospholipid (APT(EDB)-PEG(2000)-DSPE). A nanoemulsion method was then used to coat oleic acid-stabilized SPIONs with amphiphilic phospholipids, including APT(EDB)-PEG(2000)-DSPE, methoxy-PEG(2000)-DSPE, and rhodamine-DMPE. The resulting nanoparticles (APT(EDB)-SPIONs) had a hydrodynamic size of less than 50 nm and remained stable in an aqueous solution for at least 1 week. In in vitro studies, APT(EDB)-SPIONs showed specific uptake by EDB-overexpressing cell lines. In an in vivo Lewis lung carcinoma model that expresses a high level of the target EDB protein, MRI clearly revealed that APT(EDB)-SPIONs injected via the tail vein specifically accumulated at the tumor site. Non-targeting SPIONs lacking the anti-EDB aptide showed much lower uptake in tumor tissues than did aptide-conjugated nanoparticles. Further, we confirmed that the distribution of nanoparticles within the tumor tissue was well correlated with the areas where EDB was expressed. Our APT(EDB)-SPIONs hold high potential as a specific imaging modality for the detection of EDB-overexpressing tumors. (c) 2012 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | IRON-OXIDE NANOPARTICLES | - |
dc.subject | MULTIFUNCTIONAL MAGNETIC NANOPARTICLES | - |
dc.subject | BIOMEDICAL APPLICATIONS | - |
dc.subject | CONTRAST AGENTS | - |
dc.subject | THERAPY | - |
dc.subject | DELIVERY | - |
dc.subject | DESIGN | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | TRANSLATION | - |
dc.subject | MARKER | - |
dc.title | Fibronectin extra domain B-specific aptide conjugated nanoparticles for targeted cancer imaging | - |
dc.type | Article | - |
dc.identifier.wosid | 000311579200002 | - |
dc.identifier.scopusid | 2-s2.0-84866555295 | - |
dc.type.rims | ART | - |
dc.citation.volume | 163 | - |
dc.citation.issue | 2 | - |
dc.citation.beginningpage | 111 | - |
dc.citation.endingpage | 118 | - |
dc.citation.publicationname | JOURNAL OF CONTROLLED RELEASE | - |
dc.identifier.doi | 10.1016/j.jconrel.2012.08.029 | - |
dc.contributor.localauthor | Jon, Sangyong | - |
dc.contributor.nonIdAuthor | Park, Jinho | - |
dc.contributor.nonIdAuthor | Kim, Sunghyun | - |
dc.contributor.nonIdAuthor | Lee, In-Hyun | - |
dc.contributor.nonIdAuthor | Yu, Mi Kyung | - |
dc.contributor.nonIdAuthor | Kim, Minsik | - |
dc.contributor.nonIdAuthor | Lee, Kwangyeol | - |
dc.contributor.nonIdAuthor | Kim, Yong-Chul | - |
dc.contributor.nonIdAuthor | Jeong, Yong Yeon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Aptide | - |
dc.subject.keywordAuthor | Fibronectin extra domain | - |
dc.subject.keywordAuthor | SPION | - |
dc.subject.keywordAuthor | MRI | - |
dc.subject.keywordAuthor | Cancer imaging | - |
dc.subject.keywordPlus | IRON-OXIDE NANOPARTICLES | - |
dc.subject.keywordPlus | MULTIFUNCTIONAL MAGNETIC NANOPARTICLES | - |
dc.subject.keywordPlus | BIOMEDICAL APPLICATIONS | - |
dc.subject.keywordPlus | CONTRAST AGENTS | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | DESIGN | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | TRANSLATION | - |
dc.subject.keywordPlus | MARKER | - |
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