Effects of nutritional enrichment on the production of acetone-butanol-ethanol (ABE) by Clostridium acetobutylicum

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Clostridium acetobutylicum is an industrially important organism that produces acetone-butanol-ethanol (ABE). The main objective of this study was to characterize the effects of increased cell density on the production of ABE during the phase transition from acidogenesis to solventogenesis in C. acetobutylicum. The increased ABE productivity of C. acetobutylicum was obtained by increasing the cell density using a newly designed medium (designated C. a cetobutylicum medium 1; CAM1). The maximum OD600 value of C. acetobutylicum ATCC 824 strain obtained with CAM1 was 19.7, which is 1.8 times higher than that obtained with clostridial growth medium (CGM). The overall ABE productivity obtained in the CAM1-fermetation of the ATCC 824 strain was 0.83 g/L/h, which is 1.5 times higher than that (0.55 g/L/h) obtained with CGM. However, the increased productivity obtained with CAM1 did not result in an increase in the final ABE titer, because phase transition occurred at a high titer of acids.
Publisher
MICROBIOLOGICAL SOCIETY KOREA
Issue Date
2012-12
Language
Korean
Article Type
Article
Citation

JOURNAL OF MICROBIOLOGY, v.50, no.6, pp.1063 - 1066

ISSN
1225-8873
DOI
10.1007/s12275-012-2373-1
URI
http://hdl.handle.net/10203/102582
Appears in Collection
CBE-Journal Papers(저널논문)
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