In Vivo Tracking of Mesechymal Stem Cells Using Fluorescent Nanoparticles in an Osteochondral Repair Model

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We devised and tested an in vivo system to monitor the migration of mesenchymal stem cells (MSCs) within the marrow cavity. In vitro studies confirmed that platelet-derived growth factor (PDGF)-AA had the most potent chemotactic effect of the tested factors, and possessed the greatest number of receptors in MSCs. MSCs were labeled with fluorescent nanoparticles and injected into the marrow cavity of nude rats through osteochondral defects created in the distal femur. The defects were sealed with HCF (heparin-conjugated fibrin) or PDGF-AA-loaded HCF. In the HCF-only group, the nanoparticle-labeled MSCs dispersed outside the marrow cavity within 3 days after injection. In the PDGF-AA-loaded HCF group, the labeled cells moved time-dependently for 14 days toward the osteochondral defect. HCF-PDGF in low dose (LD; 8.5 ng/mu l) was more effective than HCF-PDGF in high dose (HD; 1 7 ng/mu l) in recruiting the MSCs to the osteochondral defect. After 21 days, the defects treated with PDGF and transforming growth factor (TGF)-beta 1-loaded HCF showed excellent cartilage repair compared with other groups. Further studies confirmed that this in vivo osteochondral MSCs tracking system (IOMTS) worked for other chemoattractants (chemokine (C-C motif) ligand 2 (CCL2) and PDGF-BB). IOMTS can provide a useful tool to examine the effect of growth factors or chemokines on endogenous cartilage repair. Received 13 December 2011; accepted 28 February 2012; advance online publication 70 April 2012. doi:10.1038/mt.2012.60
Publisher
NATURE PUBLISHING GROUP
Issue Date
2012-07
Language
English
Article Type
Article
Keywords

MESENCHYMAL PROGENITOR CELLS; BONE-MARROW; CARTILAGE REPAIR; ARTICULAR CHONDROCYTES; MAGNETIC NANOPARTICLES; VIRAL VECTORS; GENE DELIVERY; PDGF-AA; TISSUE; MIGRATION

Citation

MOLECULAR THERAPY, v.20, no.7, pp.1434 - 1442

ISSN
1525-0016
DOI
10.1038/mt.2012.60
URI
http://hdl.handle.net/10203/102420
Appears in Collection
CH-Journal Papers(저널논문)
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