Microneedle Delivery of H5N1 Influenza Virus-Like Particles to the Skin Induces Long-Lasting B- and T-Cell Responses in Mice

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A simple method suitable for self-administration of vaccine would improve mass immunization, particularly during a pandemic outbreak. Influenza virus-like particles (VLPs) have been suggested as promising vaccine candidates against potentially pandemic influenza viruses, as they confer long-lasting immunity but are not infectious. We investigated the immunogenicity and protective efficacy of influenza H5 VLPs containing the hemagglutinin (HA) of A/Vietnam/1203/04 (H5N1) virus delivered into the skin of mice using metal microneedle patches and also studied the response of Langerhans cells in a human skin model. Prime-boost microneedle vaccinations with H5 VLPs elicited higher levels of virus-specific IgG1 and IgG2a antibodies, virus-specific antibody-secreting cells, and cytokine-producing cells up to 8 months after vaccination compared to the same antigen delivered intramuscularly. Both prime-boost microneedle and intramuscular vaccinations with H5 VLPs induced similar hemagglutination inhibition titers and conferred 100% protection against lethal challenge with the wild-type A/Vietnam/1203/04 virus 16 weeks after vaccination. Microneedle delivery of influenza VLPs to viable human skin using microneedles induced the movement of CD207(+) Langerhans cells toward the basement membrane. Microneedle vaccination in the skin with H5 VLPs represents a promising approach for a self-administered vaccine against viruses with pandemic potential.
Publisher
AMER SOC MICROBIOLOGY
Issue Date
2010-09
Language
English
Article Type
Article
Keywords

PROTECTIVE IMMUNE-RESPONSES; A H5N1; AVIAN INFLUENZA; MICROFABRICATED MICRONEEDLES; HETEROSUBTYPIC IMMUNITY; INTRANASAL IMMUNIZATION; INACTIVATED VACCINES; PANDEMIC INFLUENZA; RANDOMIZED-TRIAL; HEALTHY-ADULTS

Citation

CLINICAL AND VACCINE IMMUNOLOGY, v.17, no.9, pp.1381 - 1389

ISSN
1556-6811
DOI
10.1128/CVI.00100-10
URI
http://hdl.handle.net/10203/102110
Appears in Collection
CBE-Journal Papers(저널논문)
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