DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Yeon-Mi | ko |
dc.contributor.author | Kim, Hee-Eun | ko |
dc.contributor.author | Park, Chin-Ju | ko |
dc.contributor.author | Lee, Ae-Ree | ko |
dc.contributor.author | Ahn, Hee-Chul | ko |
dc.contributor.author | Cho, Sung Jae | ko |
dc.contributor.author | Choi, Kwang-Ho | ko |
dc.contributor.author | Choi, Byong-Seok | ko |
dc.contributor.author | Lee, Joon-Hwa | ko |
dc.date.accessioned | 2013-03-12T06:55:55Z | - |
dc.date.available | 2013-03-12T06:55:55Z | - |
dc.date.created | 2012-06-15 | - |
dc.date.created | 2012-06-15 | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.134, no.11, pp.5276 - 5283 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | http://hdl.handle.net/10203/101582 | - |
dc.description.abstract | Z-DNA is produced in a long genomic DNA by Z-DNA binding proteins, through formation of two B-Z junctions with the extrusion of one base pair from each junction. To answer the question of how Z-DNA binding proteins induce B-Z transitions in CG-rich segments while maintaining the B-conformation of surrounding segments, we investigated the kinetics and thermodynamics of base-pair openings of a 13-bp DNA in complex with the Z-DNA binding protein, Z alpha(ADAR1). We also studied perturbations in the backbone of Z alpha(ADAR1) upon binding to DNA. Our study demonstrates the initial contact conformation as an intermediate structure during B-Z junction formation induced by Z alpha(ADAR1) in which the Z alpha(ADAR1) protein displays unique backbone conformational changes, but the 13-bp DNA duplex maintains the B-form helix. We also found the unique structural features of the 13-bp DNA duplex in the initial contact conformation: (i) instability of the AT-rich region II and (ii) longer lifetime for the opening state of the CG-rich region I. Our findings suggest a three-step mechanism of B-Z junction formation; (i) Z alpha(ADAR1) specifically interacts with a CG-rich DNA segment maintaining B-form helix via a unique conformation; (ii) the neighboring AT-rich region becomes very unstable, and the CG-rich DNA segment is easily converted to Z-DNA; and (iii) the AT-rich regions are base-paired again, and the B-Z junction structure is formed. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | Z-ALPHA DOMAIN | - |
dc.subject | HANDED Z-DNA | - |
dc.subject | CRYSTAL-STRUCTURE | - |
dc.subject | Z TRANSITION | - |
dc.subject | PROTON-EXCHANGE | - |
dc.subject | REVEALS | - |
dc.subject | KINETICS | - |
dc.subject | COMPLEX | - |
dc.subject | THERMODYNAMICS | - |
dc.title | NMR Study on the B-Z Junction Formation of DNA Duplexes Induced by Z-DNA Binding Domain of Human ADAR1 | - |
dc.type | Article | - |
dc.identifier.wosid | 000302191900040 | - |
dc.identifier.scopusid | 2-s2.0-84863374236 | - |
dc.type.rims | ART | - |
dc.citation.volume | 134 | - |
dc.citation.issue | 11 | - |
dc.citation.beginningpage | 5276 | - |
dc.citation.endingpage | 5283 | - |
dc.citation.publicationname | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.identifier.doi | 10.1021/ja211581b | - |
dc.contributor.localauthor | Choi, Byong-Seok | - |
dc.contributor.nonIdAuthor | Lee, Yeon-Mi | - |
dc.contributor.nonIdAuthor | Kim, Hee-Eun | - |
dc.contributor.nonIdAuthor | Park, Chin-Ju | - |
dc.contributor.nonIdAuthor | Lee, Ae-Ree | - |
dc.contributor.nonIdAuthor | Ahn, Hee-Chul | - |
dc.contributor.nonIdAuthor | Choi, Kwang-Ho | - |
dc.contributor.nonIdAuthor | Lee, Joon-Hwa | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | Z-ALPHA DOMAIN | - |
dc.subject.keywordPlus | HANDED Z-DNA | - |
dc.subject.keywordPlus | CRYSTAL-STRUCTURE | - |
dc.subject.keywordPlus | Z TRANSITION | - |
dc.subject.keywordPlus | PROTON-EXCHANGE | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | KINETICS | - |
dc.subject.keywordPlus | COMPLEX | - |
dc.subject.keywordPlus | THERMODYNAMICS | - |
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