DC Field | Value | Language |
---|---|---|
dc.contributor.author | Keem, Joo Oak | ko |
dc.contributor.author | Lee, In Hwan | ko |
dc.contributor.author | Kim, Sun Young | ko |
dc.contributor.author | Jung, Yongwon | ko |
dc.contributor.author | Chung, Bong Hyun | ko |
dc.date.accessioned | 2013-03-12T05:50:27Z | - |
dc.date.available | 2013-03-12T05:50:27Z | - |
dc.date.created | 2012-09-25 | - |
dc.date.created | 2012-09-25 | - |
dc.date.issued | 2011-12 | - |
dc.identifier.citation | BIOMATERIALS, v.32, no.34, pp.9051 - 9058 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://hdl.handle.net/10203/101472 | - |
dc.description.abstract | We introduce the strategic development of self-assembling peptide/protein fragments based on the far-red fluorescent protein mPlum. The first beta strand (mPlum 1,18 amino acids) of mPlum was engineered to spontaneously bind with the rest of the protein (mPlum 2-11, next 10 beta strands) and to form a native chromophore. The target beta strand mPlum 1 was separated from mPlum 2-11 and linked via a flexible peptide linker, resulting in fluorescently inactive circularly permuted mPlum protein (CpmPlum). In vitro evolution of this CpmPlum to a fluorescently active form and the subsequent splitting of the engineered mPlum 1 peptide afforded self-assembling mPlum fragments. Recombinantly expressed and synthetically prepared beta strand peptides were successfully assembled with the remaining mPlum protein in vitro and in cells. This developed pair of peptide/protein fragments was effectively used for peptide tag detection of alpha-synuclein in mammalian cells. Sequential expression of self-assembling mPlum fragments offered an entirely genetically encoded sensing system of naturally unfolded alpha-synuclein. (C) 2011 Elsevier Ltd. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | AMYLOID FORMATION | - |
dc.subject | COMPLEMENTATION ASSAY | - |
dc.subject | INCLUSIONS | - |
dc.subject | GFP | - |
dc.subject | AGGREGATION | - |
dc.subject | MODEL | - |
dc.title | Splitting and self-assembling of far-red fluorescent protein with an engineered beta strand peptide: Application for alpha-synuclein imaging in mammalian cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000296113500029 | - |
dc.identifier.scopusid | 2-s2.0-80053101300 | - |
dc.type.rims | ART | - |
dc.citation.volume | 32 | - |
dc.citation.issue | 34 | - |
dc.citation.beginningpage | 9051 | - |
dc.citation.endingpage | 9058 | - |
dc.citation.publicationname | BIOMATERIALS | - |
dc.identifier.doi | 10.1016/j.biomaterials.2011.08.029 | - |
dc.contributor.localauthor | Jung, Yongwon | - |
dc.contributor.nonIdAuthor | Keem, Joo Oak | - |
dc.contributor.nonIdAuthor | Lee, In Hwan | - |
dc.contributor.nonIdAuthor | Kim, Sun Young | - |
dc.contributor.nonIdAuthor | Chung, Bong Hyun | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Fluorescent protein | - |
dc.subject.keywordAuthor | Protein engineering | - |
dc.subject.keywordAuthor | Split protein | - |
dc.subject.keywordAuthor | Molecular evolution | - |
dc.subject.keywordAuthor | Biosensors | - |
dc.subject.keywordPlus | AMYLOID FORMATION | - |
dc.subject.keywordPlus | COMPLEMENTATION ASSAY | - |
dc.subject.keywordPlus | INCLUSIONS | - |
dc.subject.keywordPlus | GFP | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | MODEL | - |
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