Cartilage Oligomeric Matrix Protein-Angiopoientin-1 Enhances Angiogenesis of Isolated Islet and Maintains Normoglycemia Following Transplantation

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Islet transplantation (ITx) has potential as a therapy for patients with type 1 diabetes. For successful engraftment and insulin independence, the transplanted islets must establish an adequate, stable blood supply. Angiopoientin-1 (Ang1) is a specific growth factor that induces vascularization via the Tie2 or Tie1 receptor. In this study, we used an in vitro angiogenesis assay to evaluate islet function following transplantation and the effect of the Ang1 variant cartilage oligomeric matrix protein (COMP) Ang1 on isolated islets. The enhanced function of islets transduced with COMP-Ang1 was also confirmed in a streptozotocin (STZ)-induced diabetic mice model. In a three-dimensional collagen-based culture system, the transduction of COMP-Ang1 into islets significantly increased angiogenesis compared with the bacterial-p-galactosidase (LacZ)-transduced controls and an intact, nontransduced islet negative control group. COMP-Ang1 transduced islets also attenuated hyperglycemia in syngeneic diabetic C57BL16 mice and enhanced glucose tolerance by areas under the curves of intraperitoneal glucose tolerance tests. These findings demonstrated the capacity of COMP-Ang1 to promote revascularization in cultured islets, which may contribute to successful transplantation in vivo.
Publisher
Elsevier Science Inc
Issue Date
2010-09
Language
English
Article Type
Article
Keywords

PANCREATIC-ISLETS; BLOOD-FLOW; ANGIOPOIETIN-1 VARIANT; GLYCEMIC CONTROL; LONG-TERM; COMP-ANG1; COMP-ANGIOPOIETIN-1; MICE; LANGERHANS; INJURY

Citation

TRANSPLANTATION PROCEEDINGS, v.42, no.7, pp.2653 - 2657

ISSN
0041-1345
DOI
10.1016/j.transproceed.2010.04.048
URI
http://hdl.handle.net/10203/101353
Appears in Collection
MSE-Journal Papers(저널논문)
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