DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Young-Kue | ko |
dc.contributor.author | Koo, Tai-Young | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-12T01:34:22Z | - |
dc.date.available | 2013-03-12T01:34:22Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2009-09 | - |
dc.identifier.citation | BIOTECHNOLOGY PROGRESS, v.25, no.5, pp.1440 - 1447 | - |
dc.identifier.issn | 8756-7938 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100974 | - |
dc.description.abstract | For efficient production of native interferon-beta (IFN-beta) in recombinant CHO cell culture, the IFN-beta molecular aggregation that occurs during culture needs to be minimized. To do so, we investigated the effect of hyperosmolality and hypothermia on IFN-beta production and molecular aggregation in rCHO cell culture. Both hyperosmolality (470 mOsm/kg) and hypothermia (32 degrees C) increased specific native INF-beta productivity q(IFN-beta). Furthermore, they decreased the IFN-beta molecular aggregation, although severe IFN-beta molecular aggregation could not be avoided in the later phase of culture. To overcome growth suppression at hyperosmolality and hypothermia, cells were cultivated in a biphasic mode. Cells were first cultivated at 310 mOsm/kg and 37 degrees C for 2 days to rapidly obtain a reasonably high cell concentration. The, temperature and osmolality were then shifted to 32 degrees C and 470 mOsm/kg, respectively, to achieve high q(IFN-beta) and reduced IFN-beta molecular aggregation. Due to the enhanced q(IFN-beta) and delayed molecular aggregation, the highest native IFN-beta concentration achieved on day 6 was 18.03 +/- 0.61 mg/L, which was 5.30-fold higher than that in a control batch culture (310 mOsm/kg and 37 degrees C). Taken together, a combination of hyperosmolality and hypothermia in a biphasic culture is a useful strategy for improved native IFN-beta production from rCHO cells. (C) 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 1440-1447, 2009 | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | HAMSTER OVARY CELLS | - |
dc.subject | HUMAN FIBROBLAST INTERFERON | - |
dc.subject | KERATINOCYTE GROWTH-FACTOR | - |
dc.subject | PH | - |
dc.subject | ERYTHROPOIETIN | - |
dc.subject | STABILIZATION | - |
dc.subject | MECHANISMS | - |
dc.subject | EXPRESSION | - |
dc.subject | SUSPENSION | - |
dc.subject | REDUCTION | - |
dc.title | Enhanced Interferon-beta Production by CHO Cells Through Elevated Osmolality and Reduced Culture Temperature | - |
dc.type | Article | - |
dc.identifier.wosid | 000271950200028 | - |
dc.identifier.scopusid | 2-s2.0-70350141643 | - |
dc.type.rims | ART | - |
dc.citation.volume | 25 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 1440 | - |
dc.citation.endingpage | 1447 | - |
dc.citation.publicationname | BIOTECHNOLOGY PROGRESS | - |
dc.identifier.doi | 10.1002/btpr.234 | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Koo, Tai-Young | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CHO cells | - |
dc.subject.keywordAuthor | hyperosmolality | - |
dc.subject.keywordAuthor | hypothermia | - |
dc.subject.keywordAuthor | interferon-beta | - |
dc.subject.keywordAuthor | molecular aggregation | - |
dc.subject.keywordPlus | HAMSTER OVARY CELLS | - |
dc.subject.keywordPlus | HUMAN FIBROBLAST INTERFERON | - |
dc.subject.keywordPlus | KERATINOCYTE GROWTH-FACTOR | - |
dc.subject.keywordPlus | PH | - |
dc.subject.keywordPlus | ERYTHROPOIETIN | - |
dc.subject.keywordPlus | STABILIZATION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SUSPENSION | - |
dc.subject.keywordPlus | REDUCTION | - |
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