Bcl-x(L) overexpression delays the onset of autophagy and apoptosis in hyperosmotic recombinant Chinese hamster ovary cell cultures

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dc.contributor.authorHan, Young-Kueko
dc.contributor.authorHa, Tae-Kwangko
dc.contributor.authorKim, Yeon-Guko
dc.contributor.authorLee, Gyun-Minko
dc.date.accessioned2013-03-12T01:03:19Z-
dc.date.available2013-03-12T01:03:19Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2011-10-
dc.identifier.citationJOURNAL OF BIOTECHNOLOGY, v.156, no.1, pp.52 - 55-
dc.identifier.issn0168-1656-
dc.identifier.urihttp://hdl.handle.net/10203/100899-
dc.description.abstractHyperosmolality in recombinant Chinese hamster ovary (rCHO) cell cultures induces autophagy and apoptosis. To investigate the effect of Bcl-x(L) overexpression on autophagy and apoptosis in hyperosmotic rCHO cell cultures, an erythropoietin (EPO)-producing rCHO cell line with regulated Bcl-x(L) overexpression was subjected to hyperosmolality resulting from NaCl addition in a batch culture and nutrient supplementation in a fed-batch culture. In the batch culture, Bcl-x(L) overexpression suppressed apoptosis, as evidenced by a decreased amount of cleaved caspase-7 and PARP. Concurrently, Bcl-x(L) overexpression also delayed autophagy, as indicated by reduced LC3 conversion, from LC3-I to LC3-II. As a result, the cell viability and EPO production were improved by Bcl-x(L) overexpression. In the fed-batch culture, the simultaneous application of Bcl-x(L) overexpression and nutrient feeding increased the culture longevity and maximum EPO concentration. Taken together, Bcl-x(L) overexpression delayed autophagy and apoptosis in hyperosmotic rCHO cell cultures, resulting in increased EPO production. (C) 2011 Elsevier B.V. All rights reserved.-
dc.languageEnglish-
dc.publisherELSEVIER SCIENCE BV-
dc.subjectMONOCLONAL-ANTIBODY PRODUCTION-
dc.subjectCHO-CELLS-
dc.subjectOSMOLALITY-
dc.subjectPRESSURE-
dc.subjectJNK-
dc.subjectPHOSPHORYLATION-
dc.subjectTRANSLOCATION-
dc.subjectMITOCHONDRIA-
dc.subjectSTRESS-
dc.subjectBCL-2-
dc.titleBcl-x(L) overexpression delays the onset of autophagy and apoptosis in hyperosmotic recombinant Chinese hamster ovary cell cultures-
dc.typeArticle-
dc.identifier.wosid000295827600007-
dc.identifier.scopusid2-s2.0-80052911720-
dc.type.rimsART-
dc.citation.volume156-
dc.citation.issue1-
dc.citation.beginningpage52-
dc.citation.endingpage55-
dc.citation.publicationnameJOURNAL OF BIOTECHNOLOGY-
dc.contributor.localauthorLee, Gyun-Min-
dc.contributor.nonIdAuthorHa, Tae-Kwang-
dc.contributor.nonIdAuthorKim, Yeon-Gu-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorBcl-x(L)-
dc.subject.keywordAuthorAutophagy-
dc.subject.keywordAuthorApoptosis-
dc.subject.keywordAuthorrCHO cells-
dc.subject.keywordAuthorHyperosmolality-
dc.subject.keywordAuthorFed-batch culture-
dc.subject.keywordPlusMONOCLONAL-ANTIBODY PRODUCTION-
dc.subject.keywordPlusCHO-CELLS-
dc.subject.keywordPlusOSMOLALITY-
dc.subject.keywordPlusPRESSURE-
dc.subject.keywordPlusJNK-
dc.subject.keywordPlusPHOSPHORYLATION-
dc.subject.keywordPlusTRANSLOCATION-
dc.subject.keywordPlusMITOCHONDRIA-
dc.subject.keywordPlusSTRESS-
dc.subject.keywordPlusBCL-2-
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