DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Young-Kue | ko |
dc.contributor.author | Ha, Tae-Kwang | ko |
dc.contributor.author | Kim, Yeon-Gu | ko |
dc.contributor.author | Lee, Gyun-Min | ko |
dc.date.accessioned | 2013-03-12T01:03:19Z | - |
dc.date.available | 2013-03-12T01:03:19Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-10 | - |
dc.identifier.citation | JOURNAL OF BIOTECHNOLOGY, v.156, no.1, pp.52 - 55 | - |
dc.identifier.issn | 0168-1656 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100899 | - |
dc.description.abstract | Hyperosmolality in recombinant Chinese hamster ovary (rCHO) cell cultures induces autophagy and apoptosis. To investigate the effect of Bcl-x(L) overexpression on autophagy and apoptosis in hyperosmotic rCHO cell cultures, an erythropoietin (EPO)-producing rCHO cell line with regulated Bcl-x(L) overexpression was subjected to hyperosmolality resulting from NaCl addition in a batch culture and nutrient supplementation in a fed-batch culture. In the batch culture, Bcl-x(L) overexpression suppressed apoptosis, as evidenced by a decreased amount of cleaved caspase-7 and PARP. Concurrently, Bcl-x(L) overexpression also delayed autophagy, as indicated by reduced LC3 conversion, from LC3-I to LC3-II. As a result, the cell viability and EPO production were improved by Bcl-x(L) overexpression. In the fed-batch culture, the simultaneous application of Bcl-x(L) overexpression and nutrient feeding increased the culture longevity and maximum EPO concentration. Taken together, Bcl-x(L) overexpression delayed autophagy and apoptosis in hyperosmotic rCHO cell cultures, resulting in increased EPO production. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | MONOCLONAL-ANTIBODY PRODUCTION | - |
dc.subject | CHO-CELLS | - |
dc.subject | OSMOLALITY | - |
dc.subject | PRESSURE | - |
dc.subject | JNK | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | TRANSLOCATION | - |
dc.subject | MITOCHONDRIA | - |
dc.subject | STRESS | - |
dc.subject | BCL-2 | - |
dc.title | Bcl-x(L) overexpression delays the onset of autophagy and apoptosis in hyperosmotic recombinant Chinese hamster ovary cell cultures | - |
dc.type | Article | - |
dc.identifier.wosid | 000295827600007 | - |
dc.identifier.scopusid | 2-s2.0-80052911720 | - |
dc.type.rims | ART | - |
dc.citation.volume | 156 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 52 | - |
dc.citation.endingpage | 55 | - |
dc.citation.publicationname | JOURNAL OF BIOTECHNOLOGY | - |
dc.contributor.localauthor | Lee, Gyun-Min | - |
dc.contributor.nonIdAuthor | Ha, Tae-Kwang | - |
dc.contributor.nonIdAuthor | Kim, Yeon-Gu | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Bcl-x(L) | - |
dc.subject.keywordAuthor | Autophagy | - |
dc.subject.keywordAuthor | Apoptosis | - |
dc.subject.keywordAuthor | rCHO cells | - |
dc.subject.keywordAuthor | Hyperosmolality | - |
dc.subject.keywordAuthor | Fed-batch culture | - |
dc.subject.keywordPlus | MONOCLONAL-ANTIBODY PRODUCTION | - |
dc.subject.keywordPlus | CHO-CELLS | - |
dc.subject.keywordPlus | OSMOLALITY | - |
dc.subject.keywordPlus | PRESSURE | - |
dc.subject.keywordPlus | JNK | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | TRANSLOCATION | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | BCL-2 | - |
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