L-Arabinose pathway engineering for arabitol-free xylitol production in Candida tropicalis

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dc.contributor.authorYoon, Byoung-Hoonko
dc.contributor.authorJeon, Woo-Youngko
dc.contributor.authorShim, Woo-Yongko
dc.contributor.authorKim, Jung-Hoeko
dc.date.accessioned2013-03-11T23:08:53Z-
dc.date.available2013-03-11T23:08:53Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2011-04-
dc.identifier.citationBIOTECHNOLOGY LETTERS, v.33, no.4, pp.747 - 753-
dc.identifier.issn0141-5492-
dc.identifier.urihttp://hdl.handle.net/10203/100624-
dc.description.abstractXylose reductase (XR) is a key enzyme in biological xylitol production, and most XRs have broad substrate specificities. During xylitol production from biomass hydrolysate, non-specific XRs can reduce l-arabinose, which is the second-most abundant hemicellulosic sugar, to the undesirable byproduct arabitol, which interferes with xylitol crystallization in downstream processing. To minimize the flux from l-arabinose to arabitol, the l-arabinose-preferring, endogenous XR was replaced by a d-xylose-preferring heterologous XR in Candida tropicalis. Then, Bacillus licheniformis araA and Escherichia coli araB and araD were codon-optimized and expressed functionally in C. tropicalis for the efficient assimilation of l-arabinose. During xylitol fermentation, the control strains BSXDH-3 and KNV converted 9.9 g l-arabinose l(-1) into 9.5 and 8.3 g arabitol l(-1), respectively, whereas the recombinant strain JY consumed 10.5 g l-arabinose l(-1) for cell growth without forming arabitol. Moreover, JY produced xylitol with 42 and 16% higher productivity than BSXDH-3 and KNV, respectively.-
dc.languageEnglish-
dc.publisherSPRINGER-
dc.subjectESCHERICHIA-COLI-
dc.subjectSACCHAROMYCES-CEREVISIAE-
dc.subjectD-XYLOSE-
dc.subjectISOMERASE-
dc.titleL-Arabinose pathway engineering for arabitol-free xylitol production in Candida tropicalis-
dc.typeArticle-
dc.identifier.wosid000288548900015-
dc.identifier.scopusid2-s2.0-79952754799-
dc.type.rimsART-
dc.citation.volume33-
dc.citation.issue4-
dc.citation.beginningpage747-
dc.citation.endingpage753-
dc.citation.publicationnameBIOTECHNOLOGY LETTERS-
dc.contributor.localauthorKim, Jung-Hoe-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorArabinose-
dc.subject.keywordAuthorArabitol-
dc.subject.keywordAuthorCandida tropicalis-
dc.subject.keywordAuthorXylitol-
dc.subject.keywordAuthorXylose reductase-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusSACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusD-XYLOSE-
dc.subject.keywordPlusISOMERASE-
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