DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, Byoung-Hoon | ko |
dc.contributor.author | Jeon, Woo-Young | ko |
dc.contributor.author | Shim, Woo-Yong | ko |
dc.contributor.author | Kim, Jung-Hoe | ko |
dc.date.accessioned | 2013-03-11T23:08:53Z | - |
dc.date.available | 2013-03-11T23:08:53Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-04 | - |
dc.identifier.citation | BIOTECHNOLOGY LETTERS, v.33, no.4, pp.747 - 753 | - |
dc.identifier.issn | 0141-5492 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100624 | - |
dc.description.abstract | Xylose reductase (XR) is a key enzyme in biological xylitol production, and most XRs have broad substrate specificities. During xylitol production from biomass hydrolysate, non-specific XRs can reduce l-arabinose, which is the second-most abundant hemicellulosic sugar, to the undesirable byproduct arabitol, which interferes with xylitol crystallization in downstream processing. To minimize the flux from l-arabinose to arabitol, the l-arabinose-preferring, endogenous XR was replaced by a d-xylose-preferring heterologous XR in Candida tropicalis. Then, Bacillus licheniformis araA and Escherichia coli araB and araD were codon-optimized and expressed functionally in C. tropicalis for the efficient assimilation of l-arabinose. During xylitol fermentation, the control strains BSXDH-3 and KNV converted 9.9 g l-arabinose l(-1) into 9.5 and 8.3 g arabitol l(-1), respectively, whereas the recombinant strain JY consumed 10.5 g l-arabinose l(-1) for cell growth without forming arabitol. Moreover, JY produced xylitol with 42 and 16% higher productivity than BSXDH-3 and KNV, respectively. | - |
dc.language | English | - |
dc.publisher | SPRINGER | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | D-XYLOSE | - |
dc.subject | ISOMERASE | - |
dc.title | L-Arabinose pathway engineering for arabitol-free xylitol production in Candida tropicalis | - |
dc.type | Article | - |
dc.identifier.wosid | 000288548900015 | - |
dc.identifier.scopusid | 2-s2.0-79952754799 | - |
dc.type.rims | ART | - |
dc.citation.volume | 33 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 747 | - |
dc.citation.endingpage | 753 | - |
dc.citation.publicationname | BIOTECHNOLOGY LETTERS | - |
dc.contributor.localauthor | Kim, Jung-Hoe | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Arabinose | - |
dc.subject.keywordAuthor | Arabitol | - |
dc.subject.keywordAuthor | Candida tropicalis | - |
dc.subject.keywordAuthor | Xylitol | - |
dc.subject.keywordAuthor | Xylose reductase | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | D-XYLOSE | - |
dc.subject.keywordPlus | ISOMERASE | - |
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