Enhancement of xylitol production in Candida tropicalis by co-expression of two genes involved in pentose phosphate pathway

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dc.contributor.authorAhmad, Irshadko
dc.contributor.authorShim, Woo-Yongko
dc.contributor.authorJeon, Woo-Youngko
dc.contributor.authorYoon, Byoung-Hoonko
dc.contributor.authorKim, Jung-Hoeko
dc.date.accessioned2013-03-11T23:08:23Z-
dc.date.available2013-03-11T23:08:23Z-
dc.date.created2012-03-07-
dc.date.created2012-03-07-
dc.date.issued2012-01-
dc.identifier.citationBIOPROCESS AND BIOSYSTEMS ENGINEERING, v.35, pp.199 - 204-
dc.identifier.issn1615-7591-
dc.identifier.urihttp://hdl.handle.net/10203/100623-
dc.description.abstractThe yeast Candida tropicalis produces xylitol, a natural, low-calorie sweetener whose metabolism does not require insulin, by catalytic activity of NADPH-dependent xylose reductase. The oxidative pentose phosphate pathway (PPP) is a major basis for NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes which, respectively encodes glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "PP", than in BSXDH-3. In fermentation experiments using glycerol as a co-substrate with xylose, strain PP showed volumetric xylitol productivity of 1.25 g l(-1) h(-1), 21% higher than the rate (1.04 g l(-1) h(-1)) in BSXDH-3. This is the first report of increased metabolic flux toward PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production.-
dc.languageEnglish-
dc.publisherSPRINGER-
dc.subjectRECOMBINANT SACCHAROMYCES-CEREVISIAE-
dc.subjectD-XYLOSE-
dc.subjectBIOTECHNOLOGICAL PRODUCTION-
dc.subjectDISRUPTED MUTANT-
dc.subjectDEHYDROGENASE-
dc.subjectFERMENTATION-
dc.subjectGUILLIERMONDII-
dc.subjectBIOREACTOR-
dc.subjectINCREASE-
dc.subjectYIELD-
dc.titleEnhancement of xylitol production in Candida tropicalis by co-expression of two genes involved in pentose phosphate pathway-
dc.typeArticle-
dc.identifier.wosid000298799400028-
dc.identifier.scopusid2-s2.0-84857452254-
dc.type.rimsART-
dc.citation.volume35-
dc.citation.beginningpage199-
dc.citation.endingpage204-
dc.citation.publicationnameBIOPROCESS AND BIOSYSTEMS ENGINEERING-
dc.identifier.doi10.1007/s00449-011-0641-9-
dc.contributor.localauthorKim, Jung-Hoe-
dc.type.journalArticleArticle; Proceedings Paper-
dc.subject.keywordAuthorXylitol-
dc.subject.keywordAuthorCandida tropicalis-
dc.subject.keywordAuthorGlucose-6-phosphate dehydrogenase-
dc.subject.keywordAuthor6-Phosphogluconate dehydrogenase-
dc.subject.keywordPlusRECOMBINANT SACCHAROMYCES-CEREVISIAE-
dc.subject.keywordPlusD-XYLOSE-
dc.subject.keywordPlusBIOTECHNOLOGICAL PRODUCTION-
dc.subject.keywordPlusDISRUPTED MUTANT-
dc.subject.keywordPlusDEHYDROGENASE-
dc.subject.keywordPlusFERMENTATION-
dc.subject.keywordPlusGUILLIERMONDII-
dc.subject.keywordPlusBIOREACTOR-
dc.subject.keywordPlusINCREASE-
dc.subject.keywordPlusYIELD-
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