Enhancement of xylitol production in Candida tropicalis by co-expression of two genes involved in pentose phosphate pathway

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The yeast Candida tropicalis produces xylitol, a natural, low-calorie sweetener whose metabolism does not require insulin, by catalytic activity of NADPH-dependent xylose reductase. The oxidative pentose phosphate pathway (PPP) is a major basis for NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes which, respectively encodes glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed "PP", than in BSXDH-3. In fermentation experiments using glycerol as a co-substrate with xylose, strain PP showed volumetric xylitol productivity of 1.25 g l(-1) h(-1), 21% higher than the rate (1.04 g l(-1) h(-1)) in BSXDH-3. This is the first report of increased metabolic flux toward PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production.
Publisher
SPRINGER
Issue Date
2012-01
Language
English
Article Type
Article; Proceedings Paper
Keywords

RECOMBINANT SACCHAROMYCES-CEREVISIAE; D-XYLOSE; BIOTECHNOLOGICAL PRODUCTION; DISRUPTED MUTANT; DEHYDROGENASE; FERMENTATION; GUILLIERMONDII; BIOREACTOR; INCREASE; YIELD

Citation

BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.35, pp.199 - 204

ISSN
1615-7591
DOI
10.1007/s00449-011-0641-9
URI
http://hdl.handle.net/10203/100623
Appears in Collection
BS-Journal Papers(저널논문)
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