DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jong-Su | ko |
dc.contributor.author | Park, Hyun-Kyu | ko |
dc.contributor.author | Kang, Bong-Chul | ko |
dc.contributor.author | Ku, Renata | ko |
dc.contributor.author | Ham, Chul-Ho | ko |
dc.contributor.author | Yang, Min-Yang | ko |
dc.date.accessioned | 2013-03-11T21:06:42Z | - |
dc.date.available | 2013-03-11T21:06:42Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2011-10 | - |
dc.identifier.citation | JOURNAL OF APPLIED PHYSICS, v.110, no.8 | - |
dc.identifier.issn | 0021-8979 | - |
dc.identifier.uri | http://hdl.handle.net/10203/100283 | - |
dc.description.abstract | We demonstrate the use of a highly effective biosensor array to fulfill the requirements of high intensity, reduced nonspecific adsorption (NSA), and low sample usage. The mixed self-assembled monolayers (SAMs), consisting of methyl-terminated and methoxy-(polyethylene-glycol (PEG))-terminated silanes, were newly applied as the background layer to reduce the background NSA via wettability control. The surface was modified by a plasma process with a pattern mask. Gold nanoparticles (AuNPs) were grafted within pattern-modified regions to increase intensity and were modified with protein G variants with cysteine residues to immobilize the antibody proteins directly. The target protein samples were selectively dewetted by the high throughput wiping process, while retaining semi-contact with the substrate. The data revealed that the background NSA was significantly reduced by 78% with selective dewetting compared to the standard method. Furthermore, the peak intensity was improved 5 times by applying AuNPs as compared to that of a planar surface, and the protein requirement was significantly reduced versus the standard process. (C) 2011 American Institute of Physics. [doi:10.1063/1.3652860] | - |
dc.language | English | - |
dc.publisher | AMER INST PHYSICS | - |
dc.subject | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject | PROTEIN ADSORPTION | - |
dc.subject | SILICON SURFACES | - |
dc.subject | SOLID SUBSTRATE | - |
dc.subject | FABRICATION | - |
dc.subject | IMMOBILIZATION | - |
dc.subject | NANOMETER | - |
dc.subject | PATTERNS | - |
dc.subject | ROUGHNESS | - |
dc.subject | ADHESION | - |
dc.title | Highly effective gold nanoparticle-enhanced biosensor array on the wettability controlled substrate by wiping | - |
dc.type | Article | - |
dc.identifier.wosid | 000296519900162 | - |
dc.identifier.scopusid | 2-s2.0-80655131999 | - |
dc.type.rims | ART | - |
dc.citation.volume | 110 | - |
dc.citation.issue | 8 | - |
dc.citation.publicationname | JOURNAL OF APPLIED PHYSICS | - |
dc.identifier.doi | 10.1063/1.3652860 | - |
dc.contributor.localauthor | Yang, Min-Yang | - |
dc.contributor.nonIdAuthor | Park, Hyun-Kyu | - |
dc.contributor.nonIdAuthor | Ku, Renata | - |
dc.contributor.nonIdAuthor | Ham, Chul-Ho | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SELF-ASSEMBLED MONOLAYERS | - |
dc.subject.keywordPlus | PROTEIN ADSORPTION | - |
dc.subject.keywordPlus | SILICON SURFACES | - |
dc.subject.keywordPlus | SOLID SUBSTRATE | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | IMMOBILIZATION | - |
dc.subject.keywordPlus | NANOMETER | - |
dc.subject.keywordPlus | PATTERNS | - |
dc.subject.keywordPlus | ROUGHNESS | - |
dc.subject.keywordPlus | ADHESION | - |
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