DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Se Jin | ko |
dc.contributor.author | Shin, Gi Won | ko |
dc.contributor.author | Choi, Seok Jin | ko |
dc.contributor.author | Hwang, Hee Sung | ko |
dc.contributor.author | Jung, Gyoo Yeol | ko |
dc.contributor.author | SEO, TAE SEOK | ko |
dc.date.accessioned | 2013-03-11T15:52:41Z | - |
dc.date.available | 2013-03-11T15:52:41Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2010-03 | - |
dc.identifier.citation | ELECTROPHORESIS, v.31, pp.1108 - 1115 | - |
dc.identifier.issn | 0173-0835 | - |
dc.identifier.uri | http://hdl.handle.net/10203/99526 | - |
dc.description.abstract | Rapid and simple analysis for the multiple target pathogens is critical for patient management CE-SSCP analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields The capability of separating ssDNA molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram In this study, we explored the potential of poly(ethyleneoxide)-poly (propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) triblock copolymer as a sieving matrix for CE-SSCP analysis on a microdevice To demonstrate the superior resolving power of PEO-PPO-PEO copolymers, 255-bp PCR amplicons obtained from 16S ribosomal RNA genes of four bacterial species, namely Proteus mirabilis, Haemophilus ducreyt, Pseudomonas aerugmosa, and Neisseria meningitidis, were analyzed in the PEO-PPO-PEO matrix in comparison with 5% linear polyacrylamide and commercial GeneScan (TM) gel. Due to enhanced dynamic coating and sieving ability, PEO-PPO-PEO copolymer displayed fourfold enhancement of resolving power in the CE-SSCP to separate same-sized DNA molecules Fivefold Input of genomic DNA of P aeruginosa and/or N meningitidis produced proportionally increased corresponding amplicon peaks, enabling correct quantitative analysis in the pathogen detection. Besides the high-resolution sieving capability, a facile loading and replenishment of gel in the microchannel due to thermally reversible gelation property makes PEO-PPO-PEO triblock copolymer an excellent matrix in the CE-SSCP analysis on the microdevice | - |
dc.language | English | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | STRAND CONFORMATION POLYMORPHISM | - |
dc.subject | MICROCHIP ELECTROPHORESIS | - |
dc.subject | FOODBORNE PATHOGENS | - |
dc.subject | BLOCK-COPOLYMERS | - |
dc.subject | PCR | - |
dc.subject | SEPARATION | - |
dc.subject | SYSTEM | - |
dc.subject | GENE | - |
dc.subject | SSCP | - |
dc.subject | CE | - |
dc.title | Triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection | - |
dc.type | Article | - |
dc.identifier.wosid | 000276807300017 | - |
dc.identifier.scopusid | 2-s2.0-77949750891 | - |
dc.type.rims | ART | - |
dc.citation.volume | 31 | - |
dc.citation.beginningpage | 1108 | - |
dc.citation.endingpage | 1115 | - |
dc.citation.publicationname | ELECTROPHORESIS | - |
dc.identifier.doi | 10.1002/elps.200900651 | - |
dc.contributor.localauthor | SEO, TAE SEOK | - |
dc.contributor.nonIdAuthor | Shin, Gi Won | - |
dc.contributor.nonIdAuthor | Hwang, Hee Sung | - |
dc.contributor.nonIdAuthor | Jung, Gyoo Yeol | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | CE-SSCP | - |
dc.subject.keywordAuthor | Microdevice | - |
dc.subject.keywordAuthor | Pathogen | - |
dc.subject.keywordAuthor | Poly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide) | - |
dc.subject.keywordAuthor | Quantification | - |
dc.subject.keywordPlus | STRAND CONFORMATION POLYMORPHISM | - |
dc.subject.keywordPlus | MICROCHIP ELECTROPHORESIS | - |
dc.subject.keywordPlus | FOODBORNE PATHOGENS | - |
dc.subject.keywordPlus | BLOCK-COPOLYMERS | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | SEPARATION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | SSCP | - |
dc.subject.keywordPlus | CE | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.