Triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection

Cited 16 time in webofscience Cited 0 time in scopus
  • Hit : 369
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorKim, Se Jinko
dc.contributor.authorShin, Gi Wonko
dc.contributor.authorChoi, Seok Jinko
dc.contributor.authorHwang, Hee Sungko
dc.contributor.authorJung, Gyoo Yeolko
dc.contributor.authorSEO, TAE SEOKko
dc.date.accessioned2013-03-11T15:52:41Z-
dc.date.available2013-03-11T15:52:41Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2010-03-
dc.identifier.citationELECTROPHORESIS, v.31, pp.1108 - 1115-
dc.identifier.issn0173-0835-
dc.identifier.urihttp://hdl.handle.net/10203/99526-
dc.description.abstractRapid and simple analysis for the multiple target pathogens is critical for patient management CE-SSCP analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields The capability of separating ssDNA molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram In this study, we explored the potential of poly(ethyleneoxide)-poly (propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) triblock copolymer as a sieving matrix for CE-SSCP analysis on a microdevice To demonstrate the superior resolving power of PEO-PPO-PEO copolymers, 255-bp PCR amplicons obtained from 16S ribosomal RNA genes of four bacterial species, namely Proteus mirabilis, Haemophilus ducreyt, Pseudomonas aerugmosa, and Neisseria meningitidis, were analyzed in the PEO-PPO-PEO matrix in comparison with 5% linear polyacrylamide and commercial GeneScan (TM) gel. Due to enhanced dynamic coating and sieving ability, PEO-PPO-PEO copolymer displayed fourfold enhancement of resolving power in the CE-SSCP to separate same-sized DNA molecules Fivefold Input of genomic DNA of P aeruginosa and/or N meningitidis produced proportionally increased corresponding amplicon peaks, enabling correct quantitative analysis in the pathogen detection. Besides the high-resolution sieving capability, a facile loading and replenishment of gel in the microchannel due to thermally reversible gelation property makes PEO-PPO-PEO triblock copolymer an excellent matrix in the CE-SSCP analysis on the microdevice-
dc.languageEnglish-
dc.publisherWILEY-V C H VERLAG GMBH-
dc.subjectSTRAND CONFORMATION POLYMORPHISM-
dc.subjectMICROCHIP ELECTROPHORESIS-
dc.subjectFOODBORNE PATHOGENS-
dc.subjectBLOCK-COPOLYMERS-
dc.subjectPCR-
dc.subjectSEPARATION-
dc.subjectSYSTEM-
dc.subjectGENE-
dc.subjectSSCP-
dc.subjectCE-
dc.titleTriblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection-
dc.typeArticle-
dc.identifier.wosid000276807300017-
dc.identifier.scopusid2-s2.0-77949750891-
dc.type.rimsART-
dc.citation.volume31-
dc.citation.beginningpage1108-
dc.citation.endingpage1115-
dc.citation.publicationnameELECTROPHORESIS-
dc.identifier.doi10.1002/elps.200900651-
dc.contributor.localauthorSEO, TAE SEOK-
dc.contributor.nonIdAuthorShin, Gi Won-
dc.contributor.nonIdAuthorHwang, Hee Sung-
dc.contributor.nonIdAuthorJung, Gyoo Yeol-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorCE-SSCP-
dc.subject.keywordAuthorMicrodevice-
dc.subject.keywordAuthorPathogen-
dc.subject.keywordAuthorPoly(ethyleneoxide)-poly(propyleneoxide)-poly(ethyleneoxide)-
dc.subject.keywordAuthorQuantification-
dc.subject.keywordPlusSTRAND CONFORMATION POLYMORPHISM-
dc.subject.keywordPlusMICROCHIP ELECTROPHORESIS-
dc.subject.keywordPlusFOODBORNE PATHOGENS-
dc.subject.keywordPlusBLOCK-COPOLYMERS-
dc.subject.keywordPlusPCR-
dc.subject.keywordPlusSEPARATION-
dc.subject.keywordPlusSYSTEM-
dc.subject.keywordPlusGENE-
dc.subject.keywordPlusSSCP-
dc.subject.keywordPlusCE-
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 16 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0