Triblock copolymer matrix-based capillary electrophoretic microdevice for high-resolution multiplex pathogen detection

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Rapid and simple analysis for the multiple target pathogens is critical for patient management CE-SSCP analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields The capability of separating ssDNA molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram In this study, we explored the potential of poly(ethyleneoxide)-poly (propyleneoxide)-poly(ethyleneoxide) (PEO-PPO-PEO) triblock copolymer as a sieving matrix for CE-SSCP analysis on a microdevice To demonstrate the superior resolving power of PEO-PPO-PEO copolymers, 255-bp PCR amplicons obtained from 16S ribosomal RNA genes of four bacterial species, namely Proteus mirabilis, Haemophilus ducreyt, Pseudomonas aerugmosa, and Neisseria meningitidis, were analyzed in the PEO-PPO-PEO matrix in comparison with 5% linear polyacrylamide and commercial GeneScan (TM) gel. Due to enhanced dynamic coating and sieving ability, PEO-PPO-PEO copolymer displayed fourfold enhancement of resolving power in the CE-SSCP to separate same-sized DNA molecules Fivefold Input of genomic DNA of P aeruginosa and/or N meningitidis produced proportionally increased corresponding amplicon peaks, enabling correct quantitative analysis in the pathogen detection. Besides the high-resolution sieving capability, a facile loading and replenishment of gel in the microchannel due to thermally reversible gelation property makes PEO-PPO-PEO triblock copolymer an excellent matrix in the CE-SSCP analysis on the microdevice
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2010-03
Language
English
Article Type
Article
Keywords

STRAND CONFORMATION POLYMORPHISM; MICROCHIP ELECTROPHORESIS; FOODBORNE PATHOGENS; BLOCK-COPOLYMERS; PCR; SEPARATION; SYSTEM; GENE; SSCP; CE

Citation

ELECTROPHORESIS, v.31, pp.1108 - 1115

ISSN
0173-0835
DOI
10.1002/elps.200900651
URI
http://hdl.handle.net/10203/99526
Appears in Collection
CBE-Journal Papers(저널논문)
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