DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Boram | ko |
dc.contributor.author | Jung, In Hwan | ko |
dc.contributor.author | Kang, Mijeong | ko |
dc.contributor.author | Shim, Hong-Ku | ko |
dc.contributor.author | Woo, Han Young | ko |
dc.date.accessioned | 2013-03-11T08:46:56Z | - |
dc.date.available | 2013-03-11T08:46:56Z | - |
dc.date.created | 2012-04-30 | - |
dc.date.created | 2012-04-30 | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.134, no.6, pp.3133 - 3138 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.uri | http://hdl.handle.net/10203/98843 | - |
dc.description.abstract | We demonstrate highly sensitive and selective potassium ion detection against excess sodium ions in water, by modulating the interaction between the G-quadruplex-forming molecular beacon aptamer (MBA) and cationic conjugated polyelectrolyte (CPE). The K+-specific aptamer sequence in MBA is used as the molecular recognition element, and the high binding specificity of MBA for potassium ions offers selectivity against a range of metal ions. The hairpin-type MBA labeled with a fluorophore and quencher at both termini undergoes a conformational change (by complexation with CPEs) to either an open-chain form or a G-quadruplex in the absence or presence of K+ ions. Conformational changes of MBA as well as fluorescence (of the fluorophore in MBA) quenching or amplification via fluorescence resonance energy transfer from CPEs provide clear signal turn-off and -on in the presence or absence of ICE. The detection limit of the K+ assays is determined to be similar to 1.5 nM in the presence of 100 mM Na+ ions, which is similar to 3 orders of magnitude lower than those reported previously. The successful detection of 5'-adenosine triphosphate (ATP) with the MBA containing an ATP-specific aptamer sequence is also demonstrated using the same sensor scheme. The scheme reported herein is applicable to the detection of other kinds of G-rich aptamer-binding chemicals and biomolecules. | - |
dc.language | English | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | SWITCHING SIGNALING APTAMERS | - |
dc.subject | G-QUADRUPLEX | - |
dc.subject | LABEL-FREE | - |
dc.subject | CHEMICAL SENSORS | - |
dc.subject | OPTICAL PROBES | - |
dc.subject | DNA APTAMER | - |
dc.subject | FLUORESCENT | - |
dc.subject | POLYMERS | - |
dc.subject | WATER | - |
dc.subject | RECOGNITION | - |
dc.title | Cationic Conjugated Polyelectrolytes-Triggered Conformational Change of Molecular Beacon Aptamer for Highly Sensitive and Selective Potassium Ion Detection | - |
dc.type | Article | - |
dc.identifier.wosid | 000301161500053 | - |
dc.identifier.scopusid | 2-s2.0-84863116181 | - |
dc.type.rims | ART | - |
dc.citation.volume | 134 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 3133 | - |
dc.citation.endingpage | 3138 | - |
dc.citation.publicationname | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.identifier.doi | 10.1021/ja210360v | - |
dc.contributor.localauthor | Shim, Hong-Ku | - |
dc.contributor.nonIdAuthor | Kim, Boram | - |
dc.contributor.nonIdAuthor | Kang, Mijeong | - |
dc.contributor.nonIdAuthor | Woo, Han Young | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | SWITCHING SIGNALING APTAMERS | - |
dc.subject.keywordPlus | G-QUADRUPLEX | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | CHEMICAL SENSORS | - |
dc.subject.keywordPlus | OPTICAL PROBES | - |
dc.subject.keywordPlus | DNA APTAMER | - |
dc.subject.keywordPlus | FLUORESCENT | - |
dc.subject.keywordPlus | POLYMERS | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | RECOGNITION | - |
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