Dynamic Modeling of Lactic Acid Fermentation Metabolism with Lactococcus lactis

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A dynamic model of lactic acid fermentation using Lactococcus lactis was constructed, and a metabolic flux analysis (MFA) and metabolic control analysis (MCA) were performed to reveal an intensive metabolic understanding of lactic acid bacteria (LAB). The parameter estimation was conducted with COPASI software to construct a more accurate metabolic model. The experimental data used in the parameter estimation were obtained from an LC-MS/MS analysis and time-course simulation study. The MFA results were a reasonable explanation of the experimental data. Through the parameter estimation, the metabolic system of lactic acid bacteria can be thoroughly understood through comparisons with the original parameters. The coefficients derived from the MCA indicated that the reaction rate of L-lactate dehydrogenase was activated by fructose 1,6-bisphosphate and pyruvate, and pyruvate appeared to be a stronger activator of L-lactate dehydrogenase than fructose 1,6-bisphosphate. Additionally, pyruvate acted as an inhibitor to pyruvate kinase and the phosphotransferase system. Glucose 6-phosphate and phosphoenolpyruvate showed activation effects on pyruvate kinase. Hexose transporter was the strongest effector on the flux through L-lactate dehydrogenase. The concentration control coefficient (CCC) showed similar results to the flux control coefficient (FCC).
Publisher
KOREAN SOC MICROBIOLOGY BIOTECHNOLOGY
Issue Date
2011-02
Language
English
Article Type
Article
Keywords

NUCLEAR-MAGNETIC-RESONANCE; IN-VIVO ANALYSIS; SACCHAROMYCES-CEREVISIAE; BACTERIA; GLUCOSE; GLYCOLYSIS; TRANSPORT; YEAST

Citation

JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.21, no.2, pp.162 - 169

ISSN
1017-7825
DOI
10.4014/jmb.1007.07066
URI
http://hdl.handle.net/10203/98016
Appears in Collection
CBE-Journal Papers(저널논문)
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