As an exploratory study to investigate fatigue behavior of an elecrodeposited copper thin film under variable amplitude loading, repeated two-block loading tests were performed for various loading conditions. Plastic deformation behavior was continuously measured during the tests. Due to the load interaction effect under repeated two-block loading, fatigue life is significantly reduced. A concept is introduced to evaluate the load interaction effect and successfully utilized to predict fatigue life through simulation of the monotonic plastic strain (ratcheting strain) behavior under repeated two-block loading. Effects of load parameters of repeated two-block loading on fatigue life were also discussed. (C) 2011 Elsevier Ltd. All rights reserved.