Protein micropatterning on bifunctional organic-inorganic sol-gel hybrid materials

Active protein micropatterns and microarrays made by selective localization are popular candidates for medical diagnostics, such as biosensors, bioMEMS, and basic protein studies. In this paper, we present a simple fabrication process of thick (similar to 20 mu m) protein micropatterning using capillary force lithography with bifunctional sol-gel hybrid materials. Because bifunctional sol-gel hybrid material can have both an amine function for linking with protein and a methacryl function for photocuring, proteins such as streptavidin can be immobilized directly on thick bifunctional sol-gel hybrid micropatterns. Another advantage of the bifunctional sol-gel hybrid materials is the high selective stability of the amine group on bifunctional sol-gel hybrid patterns. Because amine function is regularly contained in each siloxane oligomers, immobilizing sites for streptavidin are widely distributed on the surface of thick hybrid micropatterns. The micropatterning processes of active proteins using efficient bifunctional sol-gel hybrid materials will be useful for the development of future bioengineered systems because they can save several processing steps and reduce costs.
Publisher
Amer Chemical Soc
Issue Date
2007-04
Language
ENG
Keywords

DIP-PEN NANOLITHOGRAPHY; PATTERNS; FABRICATION; PHOTOPOLYMERIZATION; MICROSTRUCTURES; LITHOGRAPHY; TECHNOLOGY; SURFACES; CELLS

Citation

LANGMUIR, v.23, no.9, pp.4732 - 4736

metadata.dc.identifier.alternativecitation
Langmuir, Vol. 23, No.9, pp.4732-4736
ISSN
0743-7463
URI
http://hdl.handle.net/10203/9679
Appears in Collection
MS-Journal Papers(저널논문)
  • Hit : 160
  • Download : 4
  • Cited 0 times in thomson ci
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡClick to seewebofscience_button
⊙ Cited 25 items in WoSClick to see citing articles inrecords_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0