In vivo tracking of color-coded effector, natural and induced regulatory T cells in the allograft response

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Here we present methods to longitudinally track islet allograft-infiltrating T cells in live mice by endoscopic confocal microscopy and to analyze circulating T cells by in vivo flow cytometry. We developed a new reporter mouse whose T cell subsets express distinct, 'color-coded' proteins enabling in vivo detection and identification of effector T cells (T(eff) cells) and discrimination between natural and induced regulatory T cells (nT(reg) and iT(reg) cells). Using these tools, we observed marked differences in the T cell response in recipients receiving tolerance-inducing therapy (CD154-specific monoclonal antibody plus rapamycin) compared to untreated controls. These results establish real-time cell tracking as a powerful means to probe the dynamic cellular interplay mediating immunologic rejection or transplant tolerance.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2010-06
Language
English
Article Type
Article
Keywords

TRANSPLANTATION TOLERANCE; INDUCTION; REJECTION; GENERATION; AUTOIMMUNE; APOPTOSIS; BALANCE; FOXP3

Citation

NATURE MEDICINE, v.16, no.6, pp.718 - 125

ISSN
1078-8956
DOI
10.1038/nm.2155
URI
http://hdl.handle.net/10203/96594
Appears in Collection
NT-Journal Papers(저널논문)
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