Integrated Capture, Concentration, Polymerase Chain Reaction, and Capillary Electrophoretic Analysis of Pathogens on a Chip

A laboratory-on-a-chip system for pathogen detection is presented that integrates cell preconcentration, purification, polymerase chain reaction (P(,R), and capillary electrophoretic (CE) analysis. The microdevice is composed of micropumps and valves, a cell capture structure, a 100 nL PCR reactor, and a 5 cm long CE column for amplicon separation. Sample volumes ranging from 10 to 100 mu L are introduced and driven through a fluidized bed of magnetically constrained immunomagnetic beads where the target cells are captured. After cell capture, beads are transferred using the on-chip plumps to the PCR reactor for DNA amplification. The resulting PCR products are electrophoretically injected onto the CE column for separation and detection of Escherichia coli K12 and E. coli O157 targets. A detection limit of 0.2 cfu/mu L is achieved using the E. coli O157 target and an input volume of 50 mu L. Finally, the sensitive detection of E. coli O157 in the presence of K12 at a ratio of 1:1000 illustrates the capability of our system to identify target cells in a high commensal background. This cell capture-PCR-CE microsystem is a significant advance in the development of rapid, sensitive, and specific laboratory-on-a-chip devices for pathogen detection.
Publisher
AMER CHEMICAL SOC
Issue Date
2009-05
Language
ENG
Keywords

ANALYSIS SYSTEMS; GENETIC-ANALYSIS; DNA; MICRODEVICE; PURIFICATION; MICROSYSTEM

Citation

ANALYTICAL CHEMISTRY, v.81, no.9, pp.3523 - 3528

ISSN
0003-2700
DOI
10.1021/ac900060r
URI
http://hdl.handle.net/10203/9259
Appears in Collection
CBE-Journal Papers(저널논문)
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