DC Field | Value | Language |
---|---|---|
dc.contributor.author | Koo, Deog-Bon | ko |
dc.contributor.author | Kim, Deog-Bon | ko |
dc.contributor.author | Yu, Iljung | ko |
dc.contributor.author | Kim, Ha-Na | ko |
dc.contributor.author | Lee, Kyung-Kwang | ko |
dc.contributor.author | Han, Yong Mahn | ko |
dc.date.accessioned | 2013-03-07T14:57:47Z | - |
dc.date.available | 2013-03-07T14:57:47Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2005-11 | - |
dc.identifier.citation | ANIMAL REPRODUCTION SCIENCE, v.90, no.1-2, pp.101 - 110 | - |
dc.identifier.issn | 0378-4320 | - |
dc.identifier.uri | http://hdl.handle.net/10203/90441 | - |
dc.description.abstract | The present study was to investigate the effects of in vitro fertilization conditions on in vitro development and structural integrity of pig embryos. Porcine oocytes matured in vitro were co-incubated with four different spermatozoa concentrations (0.6 x 10(5), 1.2 x 10(5), 2.5 x 10(5) and 5 x 10(5) cells/ml) for 6 h, and at a spermatozoa concentration (1.2 x 10(5) cells/ml) for 2, 4 and 6 h, respectively. Spermatozoa penetration and blastocyst formation were observed at 10 and 144 h post insemination, respectively. The allocation of a blastocyst to inner cell mass (ICM) and trophectoderm (TE) cells was determined by using a differential staining method. Polyspermy frequency increased with increasing spermatozoa concentrations. The spermatozoa-oocyte co-incubation period of 2 h provided for decreased in vitro development rate than 4 and 6h groups (P < 0.05), although no difference was detected in polyspermy frequency between spermatozoa-oocyte co-incubation periods. Interestingly, blastocysts derived from the groups with greater spermatozoa concentrations (2.5 x 10(5) and 5 x 10(5) cells/ml) had significantly fewer ICM cell nuclei as compared with those groups with lesser spermatozoa concentrations (0.6 x 10(5) and 1.2 x 10(5) cells/ml). There was no difference in the structural integrity of blastocysts among the co-incubation periods. Blastocysts derived from respective experiments were individually classified into three groups (I: < 20%; II: 20-40% and III: > 40%) based on the ratio of ICM to total cells. Proportion of blastocysts in Group II, with a presumptive normal range of structural integrity, was slightly decreased in the groups with greater spermatozoa concentrations (2.5 x 10(5) and 5 x 10(5) cells/ml). The results indicate that the spermatozoa concentration during in vitro fertilization may be important for developmental competence and quality of pig embryos. (c) 2005 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | EPIDERMAL-GROWTH-FACTOR | - |
dc.subject | INNER CELL MASS | - |
dc.subject | PORCINE OOCYTES | - |
dc.subject | CYTOPLASMIC MATURATION | - |
dc.subject | EJACULATED SPERMATOZOA | - |
dc.subject | CLONED PIGS | - |
dc.subject | SPERM | - |
dc.subject | BLASTOCYST | - |
dc.title | Effects of in vitro fertilization conditions on preimplantation development and quality of pig embryos | - |
dc.type | Article | - |
dc.identifier.wosid | 000233308200008 | - |
dc.identifier.scopusid | 2-s2.0-27644502850 | - |
dc.type.rims | ART | - |
dc.citation.volume | 90 | - |
dc.citation.issue | 1-2 | - |
dc.citation.beginningpage | 101 | - |
dc.citation.endingpage | 110 | - |
dc.citation.publicationname | ANIMAL REPRODUCTION SCIENCE | - |
dc.identifier.doi | 10.1016/j.anireprosci.2005.01.005 | - |
dc.contributor.localauthor | Han, Yong Mahn | - |
dc.contributor.nonIdAuthor | Koo, Deog-Bon | - |
dc.contributor.nonIdAuthor | Kim, Deog-Bon | - |
dc.contributor.nonIdAuthor | Yu, Iljung | - |
dc.contributor.nonIdAuthor | Kim, Ha-Na | - |
dc.contributor.nonIdAuthor | Lee, Kyung-Kwang | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | lVF | - |
dc.subject.keywordAuthor | spermatozoa concentration | - |
dc.subject.keywordAuthor | development | - |
dc.subject.keywordAuthor | structural integrity | - |
dc.subject.keywordAuthor | pig | - |
dc.subject.keywordPlus | EPIDERMAL-GROWTH-FACTOR | - |
dc.subject.keywordPlus | INNER CELL MASS | - |
dc.subject.keywordPlus | PORCINE OOCYTES | - |
dc.subject.keywordPlus | CYTOPLASMIC MATURATION | - |
dc.subject.keywordPlus | EJACULATED SPERMATOZOA | - |
dc.subject.keywordPlus | CLONED PIGS | - |
dc.subject.keywordPlus | SPERM | - |
dc.subject.keywordPlus | BLASTOCYST | - |
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