Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes

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dc.contributor.authorKoo, Deog-Bonko
dc.contributor.authorChae, Jung-Ilko
dc.contributor.authorKim, Ji-Suko
dc.contributor.authorWee, Gabbineko
dc.contributor.authorSong, Bong-Seokko
dc.contributor.authorLee, Kyung-Kwangko
dc.contributor.authorHan, Yong Mahnko
dc.date.accessioned2013-03-07T14:55:24Z-
dc.date.available2013-03-07T14:55:24Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2005-12-
dc.identifier.citationMOLECULAR REPRODUCTION AND DEVELOPMENT, v.72, no.4, pp.542 - 549-
dc.identifier.issn1040-452X-
dc.identifier.urihttp://hdl.handle.net/10203/90437-
dc.description.abstractThis study was conducted to examine the activities of maturation-promoting factor (MPF) and mitogen-activated protein (MAP) kinase in the porcine oocytes after artificial activation. To determine optimal electrical activation condition, oocytes were exposed to single DC pulse in a variety of electric field strengths (120, 150, 180, and 210 V/mm) and pulse durations (15, 30, 45, and 60 mu sec). After the artificial activation, 40-50 oocytes were cultured in a 50 mu l drop of NCSU23 medium supplemented with 0.4% BSA at 39 degrees C, 5% CO2 in air for 6 days. No difference was detected in the preimplantation development of pocine oocytes and the mean nuclei number of blastocysts between electric field strengths. Under the 180 V/mm electric field strength, short pulse durations (15 and 30 mu sec) showed a higher preimplantation developmental rate of the oocytes and mean nuclei number of blastocysts than an extended electric pulse (60 mu sec) (P<0.05). Single electrical stimulus (180 V/mm, 15 mu sec) resulted in higher preimplantation development of porcine oocytes as compared to other chemical stimulators (P<0.01). Western blot analyses showed the decrease of MPF and MAP kinase in the electrically-activated oocytes. After single electrical stimulus, the amounts of both cdc2 and ERK in porcine oocytes were remarkably reduced by 4 hr and then further decreased by 8 hr. However, the chemically-stimulated oocytes did not show any significant change at the levels of MPF and MAP kinase. Our results indicate that the optimal single electrical pulse is effective on the inactivation of MPF and MAP kinase, eventually leading to the parthenogenetic development of porcine oocytes.-
dc.languageEnglish-
dc.publisherWILEY-LISS-
dc.subjectACTIVATED PROTEIN-KINASE-
dc.subjectIN-VITRO DEVELOPMENT-
dc.subjectMATURATION-PROMOTING FACTOR-
dc.subjectBOVINE OOCYTES-
dc.subjectCHEMICAL ACTIVATION-
dc.subjectPIG OOCYTES-
dc.subjectCALCIUM IONOPHORE-
dc.subjectNUCLEAR TRANSFER-
dc.subjectMATURED INVITRO-
dc.subjectCYTOCHALASIN-B-
dc.titleInactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes-
dc.typeArticle-
dc.identifier.wosid000232812400014-
dc.identifier.scopusid2-s2.0-27144441716-
dc.type.rimsART-
dc.citation.volume72-
dc.citation.issue4-
dc.citation.beginningpage542-
dc.citation.endingpage549-
dc.citation.publicationnameMOLECULAR REPRODUCTION AND DEVELOPMENT-
dc.identifier.doi10.1002/mrd.20382-
dc.contributor.localauthorHan, Yong Mahn-
dc.contributor.nonIdAuthorKoo, Deog-Bon-
dc.contributor.nonIdAuthorChae, Jung-Il-
dc.contributor.nonIdAuthorKim, Ji-Su-
dc.contributor.nonIdAuthorWee, Gabbine-
dc.contributor.nonIdAuthorSong, Bong-Seok-
dc.contributor.nonIdAuthorLee, Kyung-Kwang-
dc.type.journalArticleArticle-
dc.subject.keywordAuthoractivation-
dc.subject.keywordAuthorsingle electrical stimulus-
dc.subject.keywordAuthorMPF-
dc.subject.keywordAuthorMAP kinase-
dc.subject.keywordAuthorporcine oocytes-
dc.subject.keywordPlusACTIVATED PROTEIN-KINASE-
dc.subject.keywordPlusIN-VITRO DEVELOPMENT-
dc.subject.keywordPlusMATURATION-PROMOTING FACTOR-
dc.subject.keywordPlusBOVINE OOCYTES-
dc.subject.keywordPlusCHEMICAL ACTIVATION-
dc.subject.keywordPlusPIG OOCYTES-
dc.subject.keywordPlusCALCIUM IONOPHORE-
dc.subject.keywordPlusNUCLEAR TRANSFER-
dc.subject.keywordPlusMATURED INVITRO-
dc.subject.keywordPlusCYTOCHALASIN-B-
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