Development of Selection Marker-free Transgenic Potato Plants with Enhanced Tolerance to Oxidative Stress

Cited 10 time in webofscience Cited 0 time in scopus
  • Hit : 362
  • Download : 0
A binary vector devoid of a plant selection-marker gene (designated as pSSA-F) was constructed to overcome bio-safety concerns about genetically modified plants. This vector carried chloroplast-targeted superoxide dismutase (SOD) and ascorbate peroxidase (APX) genes under the control of an oxidative stress-inducible (SWPA2) promoter, and was utilized to transform potato (Solanum tuberosum L.). integration of these foreign genes into transgenic plants was primarily performed via PCR with genomic DNA. Twelve marker-free transgenic lines were obtained by inoculating stem explants. The maximum transformation efficiency was 6.25% and averaged 2.2%. Successful integration of the SOD and APX genes rendered transgenic plants tolerant to methyl viologen-mediated oxidative stress at the leaf-disc and whole-plant levels. Our findings suggest that this technique for developing selection marker-free transgenic plants is feasible and can be employed with other crop species.
Publisher
Springer
Issue Date
2008-11
Language
English
Article Type
Article
Keywords

SUPEROXIDE-DISMUTASE; SWEET-POTATO; GENE; CHLOROPLASTS; TRANSFORMATION; PEROXIDASE; EXPRESSION; GROWTH; SALT; BIOSAFETY

Citation

JOURNAL OF PLANT BIOLOGY, v.51, no.6, pp.401 - 407

ISSN
1226-9239
DOI
10.1007/BF03036060
URI
http://hdl.handle.net/10203/87838
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 10 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0