Buforins: Histone H2A-derived antimicrobial peptides from toad stomach

Antimicrobial peptides (AMPs) constitute an important component of the innate immune system in a variety of organisms. Buforin I is a 39-amino acid AMP that was first isolated from the stomach tissue of the Asian toad Bufo bufo gargarizans. Buforin 11 is a 21-amino acid peptide that is derived from buforin I and displays an even more potent antimicrobial activity than its parent AMP. Both peptides share complete sequence identity with the N-terminal region of histone H2A that interacts directly with nucleic acids. Buforin I is generated from histone H2A by pepsin-directed proteolysis in the cytoplasm of gastric gland cells. After secretion into the gastric lumen, buforin I remains adhered to the mucous biofilm that lines the stomach, thus providing a protective antimicrobial coat. Buforins, which house a helix-hinge-helix domain, kill a microorganism by entering the cell without membrane permeabilization and thus binding to nucleic acids. The proline hinge is crucial for the cell penetrating activity of buforins. Buforins also are known to possess anti-endotoxin and anticancer activities, thus making these peptides attractive reagents for pharmaceutical applications. This review describes the role of buforins in innate host defense; future research paradigms: and use of these agents as human therapeutics. (C) 2008 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2009-08
Language
ENG
Keywords

HOST-DEFENSE PEPTIDES; POLYCATIONIC PEPTIDES; CATIONIC PEPTIDES; ESCHERICHIA-COLI; ANTIBACTERIAL PEPTIDE; PARASIN-I; CRYPTOSPORIDIUM-PARVUM; ANTICANCER ACTIVITY; ATLANTIC SALMON; INNATE IMMUNITY

Citation

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v.1788, no.8, pp.1564 - 1569

ISSN
0005-2736
DOI
10.1016/j.bbamem.2008.10.025
URI
http://hdl.handle.net/10203/8740
Appears in Collection
BS-Journal Papers(저널논문)
  • Hit : 345
  • Download : 3
  • Cited 0 times in thomson ci
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡClick to seewebofscience_button
⊙ Cited 52 items in WoSClick to see citing articles inrecords_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0