A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase

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To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.
Publisher
JAPAN SOC BIOSCI BIOTECHN AGROCHEM
Issue Date
2008-04
Language
English
Article Type
Article
Keywords

SUCCINIC ACID; ENHANCED PRODUCTION; EXPRESSION; GROWTH; CARBOXYLASE; ENZYMES; SYSTEM; STATE; FLUX; PCK

Citation

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.72, no.4, pp.1138 - 1141

ISSN
0916-8451
DOI
10.1271/bbb.70831
URI
http://hdl.handle.net/10203/86324
Appears in Collection
CBE-Journal Papers(저널논문)
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