Intracellular drug delivery using poly(D,L-lactide-co-glycolide) nanoparticles derivatized with a peptide from a transcriptional activator protein of HIV-1

Abstract

Biodegradable poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles were derivatized with Tat(49-57) peptide, which is the protein transduction domain from the transcriptional activator Tat protein of human immunodeficiency virus type-1 (HIV-1). The Tat(49-57) peptide-modified PLGA nanoparticles, with a mean diameter of ca. 238 nm, was effectively adsorbed on to the membrane of HaCaT cells and delivered into the nuclei without cytotoxicity.