DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, JH | ko |
dc.contributor.author | Chung, An Sik | ko |
dc.contributor.author | Steinbrenner, H | ko |
dc.contributor.author | Sies, H | ko |
dc.contributor.author | Brenneisen, P | ko |
dc.date.accessioned | 2013-03-04T10:25:10Z | - |
dc.date.available | 2013-03-04T10:25:10Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, v.423, no.1, pp.218 - 226 | - |
dc.identifier.issn | 0003-9861 | - |
dc.identifier.uri | http://hdl.handle.net/10203/82411 | - |
dc.description.abstract | Regulation of the balance of matrix metalloproteinase-2 (MMP-2) and its tissue inhibitor (TIMP-2) by thioredoxin (Trx) was investigated in human dermal fibroblasts. Expression and secretion of Trx and Trx reductase 1 (TR 1) was increased after ultraviolet (UV) A irradiation. A significant increase in proMMP-2 activity and a decrease of TIMP-2 activity in supernatants of UVA-irradiated fibroblasts were observed in gelatin and reverse zymography compared to non-irradiated fibroblasts. Removal of Trx or TR1 by immunoprecipitation diminished these changes in proMMP-2 activity. Incubation with 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB) also suppressed these changes. Incubation with recombinant Trx or TR decreased TIMP-2 activity and increased MMP-2 activity. UVA-irradiated fibroblasts, transiently transfected with a dominant-negative mutant or wild-type Trx, showed down- or upregulation of proMMP-2 activities, respectively, without significant change of protein amount. In conclusion, thioredoxin secreted by UVA irradiation is involved in the regulation of MMP-2 and TIMP-2 activities through its redox activity in human dermal fibroblasts. (C) 2003 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.publisher | Elsevier Science Inc | - |
dc.subject | MMP-3 PROTEIN-LEVELS | - |
dc.subject | B-MEDIATED INCREASE | - |
dc.subject | SKIN IN-VIVO | - |
dc.subject | INTERSTITIAL COLLAGENASE | - |
dc.subject | MESSENGER-RNA | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | SINGLET OXYGEN | - |
dc.subject | CELL INVASION | - |
dc.subject | TIMP-2 | - |
dc.subject | ACTIVATION | - |
dc.title | Thioredoxin secreted upon ultraviolet A irradiation modulates activities of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in human dermal fibroblasts | - |
dc.type | Article | - |
dc.identifier.wosid | 000188969700027 | - |
dc.identifier.scopusid | 2-s2.0-1042302126 | - |
dc.type.rims | ART | - |
dc.citation.volume | 423 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 218 | - |
dc.citation.endingpage | 226 | - |
dc.citation.publicationname | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | - |
dc.contributor.nonIdAuthor | Oh, JH | - |
dc.contributor.nonIdAuthor | Steinbrenner, H | - |
dc.contributor.nonIdAuthor | Sies, H | - |
dc.contributor.nonIdAuthor | Brenneisen, P | - |
dc.type.journalArticle | Article; Proceedings Paper | - |
dc.subject.keywordAuthor | UVA irradiation | - |
dc.subject.keywordAuthor | thioredoxin | - |
dc.subject.keywordAuthor | thioredoxin reductase | - |
dc.subject.keywordAuthor | TIMP-2 | - |
dc.subject.keywordAuthor | MMP-2 | - |
dc.subject.keywordAuthor | fibroblast | - |
dc.subject.keywordPlus | MMP-3 PROTEIN-LEVELS | - |
dc.subject.keywordPlus | B-MEDIATED INCREASE | - |
dc.subject.keywordPlus | SKIN IN-VIVO | - |
dc.subject.keywordPlus | INTERSTITIAL COLLAGENASE | - |
dc.subject.keywordPlus | MESSENGER-RNA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | SINGLET OXYGEN | - |
dc.subject.keywordPlus | CELL INVASION | - |
dc.subject.keywordPlus | TIMP-2 | - |
dc.subject.keywordPlus | ACTIVATION | - |
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