Thioredoxin secreted upon ultraviolet A irradiation modulates activities of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in human dermal fibroblasts

Cited 43 time in webofscience Cited 0 time in scopus
  • Hit : 302
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorOh, JHko
dc.contributor.authorChung, An Sikko
dc.contributor.authorSteinbrenner, Hko
dc.contributor.authorSies, Hko
dc.contributor.authorBrenneisen, Pko
dc.date.accessioned2013-03-04T10:25:10Z-
dc.date.available2013-03-04T10:25:10Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2004-
dc.identifier.citationARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, v.423, no.1, pp.218 - 226-
dc.identifier.issn0003-9861-
dc.identifier.urihttp://hdl.handle.net/10203/82411-
dc.description.abstractRegulation of the balance of matrix metalloproteinase-2 (MMP-2) and its tissue inhibitor (TIMP-2) by thioredoxin (Trx) was investigated in human dermal fibroblasts. Expression and secretion of Trx and Trx reductase 1 (TR 1) was increased after ultraviolet (UV) A irradiation. A significant increase in proMMP-2 activity and a decrease of TIMP-2 activity in supernatants of UVA-irradiated fibroblasts were observed in gelatin and reverse zymography compared to non-irradiated fibroblasts. Removal of Trx or TR1 by immunoprecipitation diminished these changes in proMMP-2 activity. Incubation with 5,5'-dithio-bis-2-nitrobenzoic acid (DTNB) also suppressed these changes. Incubation with recombinant Trx or TR decreased TIMP-2 activity and increased MMP-2 activity. UVA-irradiated fibroblasts, transiently transfected with a dominant-negative mutant or wild-type Trx, showed down- or upregulation of proMMP-2 activities, respectively, without significant change of protein amount. In conclusion, thioredoxin secreted by UVA irradiation is involved in the regulation of MMP-2 and TIMP-2 activities through its redox activity in human dermal fibroblasts. (C) 2003 Elsevier Inc. All rights reserved.-
dc.languageEnglish-
dc.publisherElsevier Science Inc-
dc.subjectMMP-3 PROTEIN-LEVELS-
dc.subjectB-MEDIATED INCREASE-
dc.subjectSKIN IN-VIVO-
dc.subjectINTERSTITIAL COLLAGENASE-
dc.subjectMESSENGER-RNA-
dc.subjectESCHERICHIA-COLI-
dc.subjectSINGLET OXYGEN-
dc.subjectCELL INVASION-
dc.subjectTIMP-2-
dc.subjectACTIVATION-
dc.titleThioredoxin secreted upon ultraviolet A irradiation modulates activities of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in human dermal fibroblasts-
dc.typeArticle-
dc.identifier.wosid000188969700027-
dc.identifier.scopusid2-s2.0-1042302126-
dc.type.rimsART-
dc.citation.volume423-
dc.citation.issue1-
dc.citation.beginningpage218-
dc.citation.endingpage226-
dc.citation.publicationnameARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS-
dc.contributor.nonIdAuthorOh, JH-
dc.contributor.nonIdAuthorSteinbrenner, H-
dc.contributor.nonIdAuthorSies, H-
dc.contributor.nonIdAuthorBrenneisen, P-
dc.type.journalArticleArticle; Proceedings Paper-
dc.subject.keywordAuthorUVA irradiation-
dc.subject.keywordAuthorthioredoxin-
dc.subject.keywordAuthorthioredoxin reductase-
dc.subject.keywordAuthorTIMP-2-
dc.subject.keywordAuthorMMP-2-
dc.subject.keywordAuthorfibroblast-
dc.subject.keywordPlusMMP-3 PROTEIN-LEVELS-
dc.subject.keywordPlusB-MEDIATED INCREASE-
dc.subject.keywordPlusSKIN IN-VIVO-
dc.subject.keywordPlusINTERSTITIAL COLLAGENASE-
dc.subject.keywordPlusMESSENGER-RNA-
dc.subject.keywordPlusESCHERICHIA-COLI-
dc.subject.keywordPlusSINGLET OXYGEN-
dc.subject.keywordPlusCELL INVASION-
dc.subject.keywordPlusTIMP-2-
dc.subject.keywordPlusACTIVATION-
Appears in Collection
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 43 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0