Isolation of neuronal precursors from differentiating P19 embryonal carcinoma cells by neuronal T alpha 1-promoter-driven GFP

Cited 4 time in webofscience Cited 0 time in scopus
  • Hit : 325
  • Download : 0
The induction of pluripotent P19 embryonal carcinoma (EC) cells with retinoic acid results in their differentiation into cells that resemble neurons, glia, and fibroblasts. To isolate and enrich the developing neurons from heterogeneously differentiating P19 EC cells, we used a recently introduced protocol combining the expression of green fluorescent protein (GFP) driven by a tissue-specific promoter and fluorescence-activated cell sorting. Cells were transfected with the gene for GFP, which is under the control of the neuronal Tal tubulin promoter. After four days of retinoic acid treatment, GFP was specifically detected in cells undergoing neuronal differentiation. Sorting of fluorescent differentiating P19 EC transfectants yielded populations highly enriched in neuronal precursors and neurons. Immunoreactivity for nestin and neurofilament was observed in 80 and 25% of the sorted cell population, respectively. These results demonstrate that differentiated neuronal precursor cells can be efficiently isolated from differentiating pluripotent embryonic cells in vitro, suggesting that this method can reproducibly provide homogeneous materials for further studies on neurogenesis. (C) 2001 ISDN. Published by Elsevier Science Ltd. All rights reserved.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Issue Date
2001-11
Language
English
Article Type
Article
Keywords

IN-VITRO; PROGENITOR CELLS; STEM-CELLS; TUBULIN PROMOTER; NERVOUS-SYSTEM; T-ALPHA-1; GENE; ZONE; REGENERATION; NEUROGENESIS

Citation

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, v.19, pp.631 - 638

ISSN
0736-5748
DOI
10.1016/S0736-5748(01)00049-1
URI
http://hdl.handle.net/10203/81888
Appears in Collection
MSE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 4 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0