Development of quartz crystal microbalance-based immunosensor for the determination of low-density lipoprotein Quartz Crystal Microbalance 시스템을 이용한 저밀도 지질단백질측정용 면역센서의 개발

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Immunosensor for the determination of LDL(Low-Density Lipoprotein), a good indicator for the diagnosis of atherosclerosis and hypercholesterolemia, was developed by using quartz crystal microbalance(QCM). The immunosensor consists of flow-through cell, oscillating circuit, oscilloscope, and frequency counter. FIA(Flow Injection Analysis) was applied to the QCM system for the measurement of LDL in liquid phase. Antibody showing binding affinity against LDL was immobilized on the gold electrode of a quartz crystal by covalent coupling via polyethylenimine / glutaredehyde. LDL was injected and bound to the antibody immobilized on the QCM immunosensor. The response of the immunosensor (F0 - F1) was found to be proportional to the LDL concentration from 200 ㎍/ml to 800 ㎍/ml. Operational conditions for the operation of immunosensor were also investigated in terms of sensitivity and non-specific binding.
Publisher
한국생물공학회
Issue Date
1998-04
Language
Korean
Citation

KSBB JOURNAL, v.13, no.4, pp.337 - 342

ISSN
1225-7117
URI
http://hdl.handle.net/10203/77539
Appears in Collection
BS-Journal Papers(저널논문)
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