Development of a serum-free medium for the production of humanized antibody from Chinese hamster ovary cells using a statistical design

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To develop serum-free (SF) media for the production of humanized antibody from recombinant Chinese hamster ovary (rCHO cells, a statistical optimization approach based on a Plackett-Burman design was adopted. A basal medium was prepared by supplementing alpha-minimal essential medium (alpha-MEM) with Fe(NO3)(3). 9H(2)O, CuCl2, ZnSO4. 7H(2)O, and Na2SeO3 which are generally contained in SF medium formulations. Insulin, transferrin, and ethanolamine were also supplemented to the basal medium to determine their optimal concentrations. From this statistical analysis, serine, phenylalanine, and tyrosine were identified as important determinants for cell growth. Also, putrescine, linoleic acid, and hydrocortisone were shown to be important for both cell growth and antibody production. The SF medium was formulated by supplementing the basal medium with components showing positive effects on cell growth and/or antibody production. Cell growth and antibody production in this SF medium were comparable to those in alpha-MEM supplemented with 5% dialyzed fetal bovine serum. Taken together, the results obtained here show that a Plackett-Burman design facilitates the development of SF media for rCHO cells aimed at producing a humanized antibody.
Publisher
SOC IN VITRO BIOLOGY
Issue Date
1998
Language
English
Article Type
Article
Keywords

INTERFERON-GAMMA PRODUCTION; PRODUCTION KINETICS; CULTURE-MEDIUM; GROWTH; OPTIMIZATION; HYBRIDOMA; LINE

Citation

IN VITRO CELLULAR DEVELOPMENTAL BIOLOGY-ANIMAL, v.34, no.10, pp.757 - 761

ISSN
1071-2690
URI
http://hdl.handle.net/10203/77350
Appears in Collection
BS-Journal Papers(저널논문)
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