Optimization of culture conditions for production of a high viscosity polysaccharide, methylan, by methylobacterium organophilum from methanol

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An extracellular polysaccharide, methylan, was produced under the specific conditions by Methylobacteriurn organophilum from methanol. The specific growth rate of cells was approximately constant regardless of C/N ratio and the specific product yield was maximum at a C/N ratio of 30. Methylan production was suppressed by the deficiency of mineral ions such as Mn** or Fe** ion. The optimal pH for cell growth and methylan production was 7. Whereas the optimal temperature for cell growth was found to be 37°C, that for methylan production was 30°C. The methanol concentration above 4% completely inhibited the cell growth. The initial methanol concentration for the maximal production of methylan was 0.5% (v/v) and above this concentration, methylan production was markedly inhibited. To overcome the substrate toxicity and inhibition for both cell growth and methylan production, a fed-bach culture of intermittent feeding within 5 g/l methanol was conducted under the optimal culture condition. Methylan production of was stimulated by nitrogen limitation and methylan was accumulated up to 8.7 g/l and cell mass also increased up to 12.4 g/l.
Publisher
Korean Society for Applied Microbiology
Issue Date
1998-06
Language
English
Citation

KOREAN JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.26, no.3, pp.244 - 249

ISSN
0257-2389
URI
http://hdl.handle.net/10203/77283
Appears in Collection
BS-Journal Papers(저널논문)
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