Justification of continuous packed-bed reactor for retroviral vector production from amphotropic Psi CRIP murine producer cell

Cited 12 time in webofscience Cited 0 time in scopus
  • Hit : 300
  • Download : 0
To indentify a plausible large-scale production system for retroviral vector, three culture systems, i.e., batch culture with medium exchange, microcarrier culture, and packed-bed reactor culture were compared. In batch cultures with medium exchange, high cell concentrations were maintained for about a month, and the harvested retroviral titer remained constant. In microcarrier cultures, although cell growth was rapid, the retroviral titer was unexpectedly low, suggesting that the low titer was due either to serious damage to the retroviral vector or to a reduction in the production rate of retroviral vector, caused by mechanical shear forces. Although the retroviral titer (maximum titer, 1.56 x 10(6)) in the packed-bed reactor was a little bit lower than that obtained in the batch culture with medium exchange (maximum titer, 1.91 x 10(6)), continuous production made it possible to increase the cumulative titer up to 16-fold of that from the batch culture with medium exchange. Moreover, as the packed-bed reactor system requires less labor and shows excellent volumetric productivity in comparison to batch cultures with medium exchanges, it will be an appropriate production system for retroviral vector in large quantities.
Publisher
KLUWER ACADEMIC PUBL
Issue Date
2000-10
Language
English
Article Type
Article
Keywords

GENE-THERAPY; MICROCARRIERS; BIOREACTOR; CULTURES; KINETICS; TITERS; RATES; VIRUS; LINES

Citation

CYTOTECHNOLOGY, v.34, no.1-2, pp.151 - 158

ISSN
0920-9069
URI
http://hdl.handle.net/10203/75752
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 12 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0