Degradation of chloronitrobenzenes by a coculture of Pseudomonas putida and a Rhodococcus sp.

A single microorganism able to mineralize chloronitrobenzenes (CNBs) has not been reported, and degradation of CNBs by coculture of two microbial strains was attempted. Pseudomonas putida HS12 was first isolated by analogue enrichment culture using nitrobenzene (NB) as the substrate, and this strain was observed to possess a partial reductive pathway for the degradation of NE. From high-performance liquid chromatography-mass spectrometry and H-1 nuclear magnetic resonance analyses, NB-grown cells of P. putida HS12 were found to convert 3- and 4-CNBs to the corresponding 5- and 4-chloro-2-hydroacetanilides, respectively, by partial reduction and subsequent acetylation, For the degradation of CNBs, Rhodococcus sp, strain HS51, which degrades 4- and 5-chloro-2-hydroxyacetanilides, was isolated and combined with P. putida HS12 to give a coculture. This coculture was confirmed to mineralize 3- and 4-CNBs in the presence of an additional carbon source. A degradation pathway for 3- and 4-CNBs by the two isolated strains was also proposed.
Publisher
AMER SOC MICROBIOLOGY
Issue Date
1999-03
Language
ENG
Keywords

META-CLEAVAGE PATHWAY; MICROBIAL-DEGRADATION; AEROBIC CONDITIONS; HYBRID PATHWAY; BIODEGRADATION; NITROBENZENE; STRAIN; PSEUDOALCALIGENES; 4-NITROTOLUENE; CATABOLISM

Citation

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.65, no.3, pp.1083 - 1091

ISSN
0099-2240
URI
http://hdl.handle.net/10203/75052
Appears in Collection
BS-Journal Papers(저널논문)
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